DNA RNA Nucleic Acid PCR Detection Pfu PCR Master Mix P2021 1ml

Pfu Mix

Pfu Mix for DNA RNA Nucleic Acid PCR Detection P2021 1ml

#P2022, 1 ml

Contents:

Description

2× Pfu Mix is a premixed, ready-to-use solution containing Pfu DNA Polymerase, dNTPs, Mg²⁺ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. This premixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set-up. It also contributes to higher sensitivity by adding enhancer.

Pfu DNA Polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has been shown to exhibit superior thermostability and proofreading properties compared to other thermostable polymerase. Unlike Taq DNA Polymerase, highly thermostable Pfu DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA Polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA Polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis. The elongation velocity is 1kb/min(70~75°C).

Composition of the 2× Pfu Mix

0.15U/μl Pfu DNA Polymerase, 2× Pfu Buffer, 0.4mM dNTPs, 4mM MgSO_4, 0.02% bromophenol blue.

Pfu Mix buffer is a proprietary formulation optimized for robust performance in PCR.

Applications

• High fidelity PCR

• Routine PCR with high reproducibility

• Site-directed mutagenesis

• Generation of PCR products for TA cloning

Features

• Convenient: only primers and template DNA are added when prepare final PCR

• High yields of PCR products with minimal optimization

• Fast: saves time due to reduced number of pipetting steps.

• Reproducible: lower contamination and pipetting error risk

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.