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HS QPCR Mastermix P2081 1ml High Reproducibility

Guangzhou Dongsheng Biotech Co., Ltd

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HS QPCR Mastermix P2081 1ml High Reproducibility

Country/Region china
City & Province guangzhou guangdong
Categories Dyestuff Intermediates
InquireNow

Product Details

HSTM Mix

#P2081, 1 ml

HS Mix PCR Master Mix P2081/1ml

 

Contents:

HSTM Mix1 ml
Nuclease-free water1 ml

 

Store at -20°C

For research use only.

In total 2 vials.

 

Description

HSTM Mix is a premixed, ready-to-use solution containing HSTM Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. HSTM Mix contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. It also contributes to higher specificity by optimizing the system, reducing primer-dimer rate.

HSTM Taq DNA Polymerase is a thermostable recombinant DNA Polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. HSTM Taq DNA Polymerase can amplify DNA target up to 5 kb. The elongation velocity is 2kb/min. It has 5' to 3' polymerase activity, but lacks of 3' to 5' exonuclease activity that results in 3'-dA overhangs PCR products. All components of the HSTM Mix are at optimal concentration for efficient amplification. It contributes to highly specific incorporation of primers and template.

Composition of the 2× HSTM Mix

0.3U/µl HSTM Taq DNA Polymerase, 2× HSTM PCR Buffer, 0.4mM dNTPs, 3.2mM MgCl2, 0.02% bromophenol blue.

HSTM Mix buffer is a proprietary formulation optimized for robust performance in PCR.

Applications

• High throughput PCR

• High specificity PCR

• Routine PCR with high reproducibility

• Generation of PCR products for TA cloning

Features

• Convenient: only primers and template DNA are added

when prepare final PCR

• High yields of PCR products with minimal optimization

• High efficiency: saving your time by simplifying the process

• Reproducible: lower contamination and pipetting error risk

• High sensitivity: compared to conventional Taq DNA Polymerase.

 

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.

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