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500bp Ladder
Concentration: 92ng/µl
Components
Component | M1251 | M1252 |
500bp Ladder | 50ug | 5x50ug |
Recommended Loading
3-5 µl/Lane.
Concentration
Typical Bands 100 ng/5µl
Other Bands 40 ng/5µl
Recommended Electrophoresis Condition
5 µl/Lane, 8 cm 1%Agarose Gel, 1×TAE, 7V/cm, 45min
Contents(bp)
500,1000,1500,2000,2500,3000,3500,4000,4500,5000
Storage
1 year at room temperature, for long-time preservation, please storage at -20°C .
Description
500bp ladder is ideal for determining the size of double-stranded DNA from 500 to 5,000 base pairs. The ladder consists of 10 linear double-stranded fragments .The 2,500bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 ul loading, all fragments except 2,000bp are 40ng. The 2,000bp fragment is 100ng. This ladder is pre-mixed with loading dye and is ready to use.
Recommendations
Do not heat before loading.
For quantification, adjust the concentration of the sample to equalize
it approximately with the amount of DNA in the nearest band of the ladder.
Visualize DNA by staining with ethidium bromide or with SYBR® Green I.
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