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[China]
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Address: Room 305, Building A, No. 179, Guangpu East Road, Huangpu District, Guangzhou, Guangdong
Contact name:
Guangzhou Dongsheng Biotech Co., Ltd |
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Verified Suppliers
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Instruction for Use
【Product Name】
Fast DNA Library Plus Prep Kit for MGI
【Cat. No./Spec.】
KM004-A/24 rxns; KM004-B/96 rxns; sample sack/ 6 rxns
【Product Description】
Aiming at MGI high-throughput sequencing platform, this kit provides a convenient and universal DNA library construction scheme in one tube. It combines fragmentation, end repair and A-Tailing into one step, greatly shortening the time of library construction and reducing the error caused by tedious steps. After fragmentation and end preparation, the product can be directly ligated with adapter without additional purification, and the subsequent procedure is the same as that of #KM001 Fast DNA Library Prep Kit for MGI. Complete library quantification can be performed by dsDNA fluorescent dye method (e.g., Thermo Qubit Flex Fluorometer) or absolute quantification PCR after diluting the library to an appropriate concentration.
【Sample Type】
Table 1 Recommended Inputs for Common DNA
| Application | Sample Type | Recommended Amount |
| Whole genome sequencing | High quality complex genomes | 50ng-1μg |
| Target capture sequencing of whole exome | High quality complex genomes | 10ng-1μg |
| Target capture sequencing of whole genome | FFPE DNA | ≥50ng |
| Whole genome sequencing | Microbial genome | 1ng-1μg |
【Storage Condition & Shelf Life】
All reagents should be stored at -20°C. Ligation Buffer is normal for crystals to precipitate at low temperatures, it should be balanced to room temperature before use. The product is valid for 12 months.
【Components】
| Component | 24 rxns | 96 rxns |
| FEP Buffer | 120 μl | 480 μl |
| FEP Enzyme Mix | 240 μl | 2×480 μl |
| Fast DNA Ligase | 120 μl | 2×240 μl |
| Fast Ligation Buffer | 600 μl | 4×600 μl |
| 2× HIFI Library PCR Master Mix | 600 μl | 4×600 μl |
| Primer Mix for MGI * | 120 μl | 480 μl |
| Neutralization Buffer | 120 μl | 480 μl |
*FEP Buffer is the fragmentation and end preparation reaction buffer. FEP Enzyme Mix is a mixture of enzyme related to fragmentation and end preparation.
* If there are more than one sample, #KM002 and #KM003 adapter primer mix is recommended. This kit provides a set of primers, the primer sequence is as follows:
5’-TGTGAGCCAAGGAGTTG-3’
5’-GAACGACATGGCTACGA-3’
Note:recommended selection beads: #NC1011 GDSPure DNA Selection Magbeads or AMPure XP beads.
GDSBio Is a high-tech enterprise focusing on research and development, production and sales of high-quality life science products. The company has a complete product line, with PCR technology as the core, focusing on general PCR, fluorescence quantitative PCR, NGS storage, nucleic acid electrophoresis and other molecular biology technologies, and has developed molecular research reagents, molecular in vitro diagnostic raw materials, nucleic acid extraction and detection reagents and other products.
