GDSBio Power Green Master Mixture For PCR With ROX P2101c P2102c

Cat. No: P2101c, 400 rxn/20μl reaction

P2102c, 2000 rxn/20μl reaction

Power Green qPCR Mix with ROX

Power Green qPCR Mix with ROX P2101c/P2102c

For research use only

Components

* ROX Reference Dye H and ROX Reference Dye L are suitable for fluorescence quantitative PCR instruments requiring high and low concentrations of ROX calibration respectively. It is recommended that 40 μL 50X ROX Reference Dye H or L be premixed into 1ml 2X SYBR Green qPCR Mix. The final working concentration of ROX is 1X.

Storage

This mix can be stored for 2 months at 4°C and protected from light. For longer storage, it should be kept at -20°C and protected from light. The ROX Reference Dye should be kept at -20°C and protected from light.

Description

Power Green qPCR Mix is a ready-to-use, 2X concentrated mix that contains all the reagents (except template and primers) needed for the real-time qPCR in the SYBR Green I detection format. This product is compatible with most manufacturers' real-time fluorescent quantitative PCR instruments such as Applied Biosystems, Roche, Bio-Rad, Eppendorf, Corbett and so on.

The Hotstart Taq DNA Polymerase in the mix is chemically modified, and it can reduce non-specific amplification more effectively than the antibody-modified polymerase. At the same time, the mix contains aptamer-based inhibitors, which reduce the generation of the primer dimer and secondary metabolites. When the temperature below 45°C, the activity of the modified polymerase will be strongly suppressed, only when the temperature reached 72°C, it can be activated. This dual hot-start technique brings greater efficiency and specificity to PCR.

Applications

• Gene expression analysis

• Low-copy gene detection

• Microarray validation

• Gene knockdown validation

Features

• Compatible with many Real-time systems whether requires ROX reference dye to calibration or not

• Exceptional specificity with dual hot-start mechanism

• Tight reproducibility in Ct values over a broad dynamic range

• Universal instrument compatibility

Table of Instrument Guide

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.