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REAGEN™ Doxycycline ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of doxycycline in egg, honey, meat, fish , shrimp and milk.
High recovery (75-120%), rapid and cost-effective extraction methods.
High sensitivity (0.1ng/g or ppb) and low detection limit for samples.
High reproducibility.
A quick ELISA assay (less than 1 hours regardless of number of samples).
The method is based on a competitive colorimetric ELISA assay. The doxycycline-BSA has been coated in the plate wells. During the analysis, sample and the doxycycline antibody are added along with secondary antibody, tagged with a peroxidase enzyme. If the doxycycline residue is present in the sample, it will compete for the doxycycline antibody, thereby preventing the antibody from binding to the doxycycline-BSA attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the doxycycline residue concentration in the sample.
REAGEN™ Doxycycline ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused micro-wells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C . The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| Doxycycline-Coated Plate | 1 x 96-well plate (8 wells x 12 strips) | 2-8℃ |
Doxycycline Standards: Negative control (white Cap tube) 0.1ng/mL (yellow Cap tube) 0.3 ng/mL (orange Cap tube) 0.9 ng/mL (pink Cap tube) 2.7 ng/mL (purple Cap tube) 8.1 ng/mL (blue Cap tube) 500ng/mL (Spiking, red Cap tube) |
1.0 mL 1.0 mL 1.0 mL 1.0mL 1.0 mL 1.0 mL 1.0 mL |
2-8℃
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| Doxycycline Antibody #1 | 6 mL | 2-8℃ |
| HRP-Conjugated Ab #2 | 6 mL | |
| 20X Wash Solution | 30 mL | |
| 10X Sample Ext. Buffer | 30 mL | |
| Stop Buffer | 12 mL | |
| TMB Substrate | 12 mL |
If you are not planning to use the kit for over 1 month, storing Doxycycline Standard Stock, Doxycycline Antibody #1 and HRP-Conjugated Ab #2 at －20℃ or in a freezer is recommended.
| Sample Type | Detection Limit (ng/g or ppb) |
| Egg | 1.5 |
| Honey/Milk | 1 |
| Meat/Fish/Shrimp | 1 |
| Analytes | Cross-Reactivity (%) |
| Doxycycline | 100 |
| Chlortetracycline | 24 |
| Oxytetracycline | 21 |
| Tetracycline | 15 |
