Active Member
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[China]
Address: #1008.Junjiang Internatioanl Bldg. 228 Ningguo Rd. Yangpu Dist. Shanghai. 200090. China
Contact name:
Shanghai Korain Biotech Co., Ltd |
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Specificity Porcine Matrix Metalloproteinase 3 ELISA Kit 96 wells
Cat.No E0455Po
Reagent Provided
| Components | Quantity |
| Standard Solution (96ng/ml) | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (30x) | 20ml x1 |
| Biotinylated Porcine MMP-3 Antibody | 1ml x1 |
| User Instruction | 1 |
| Plate Sealer | 2 pics |
| Zipper bag | 1 pic |
Reagent Preparation
All reagents should be brought to room temperature before use.
Standard Reconstitute the 120μl of the standard (96ng/ml) with 120μl of standard diluent to generate a 48ng/ml standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (48ng/ml) 1:2 with standard diluent to produce 24ng/ml, 12ng/ml, 6ng/ml and 3ng/ml solutions. Standard diluent serves as the zero standard(0 ng/ml). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:
| 48ng/ml | Standard No.5 | 120μl Original Standard + 120μl Standard Diluent |
| 24ng/ml | Standard No.4 | 120μl Standard No.5 + 120μl Standard Diluent |
| 12ng/ml | Standard No.3 | 120μl Standard No.4 + 120μl Standard Diluent |
| 6ng/ml | Standard No.2 | 120μl Standard No.3 + 120μl Standard Diluent |
| 3ng/ml | Standard No.1 | 120μl Standard No.2 + 120μl Standard Diluent |
| Standard Concentration | Standard No.5 | Standard No.4 | Standard No.3 | Standard No.2 | Standard No.1 |
| 96ng/ml | 48ng/ml | 24ng/ml | 12ng/ml | 6ng/ml | 3ng/ml |
