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RUO Helicobacter pylori (H.P) AntigenELISA Quantitative Test Kit (Feces)
This H.P ELISA Test Kit is a quantitative detection of H.P antigen in human feces, which is based on Enzyme Linked Immunosorbent method. The kit is suitable for research of Helicobacter pylori infection.
MAIN COMPONENTS
| Components | 96T/Box | |
| Coated Microtiter Plate | 1 bag | 96 wells |
| H.P reference material S0(0ng/mL) | 1 vial | 0.5ml |
| H.P reference material S1(10ng/mL) | 1 vial | 0.5ml |
| H.P reference material S2(20ng/mL) | 1 vial | 0.5ml |
| H.P reference material S3(50ng/mL) | 1 vial | 0.5ml |
| H.P reference material S4(100ng/mL) | 1 vial | 0.5ml |
| H.P reference material S5(200ng/mL) | 1 vial | 0.5ml |
| Conjugate | 1 vial | 6.0 mL |
| Control low value (24.5~45.5ng/mL) | 1 vial | 0.5ml |
| Control high value(49~91ng/mL) | 1 vial | 0.5ml |
| Wash Buffer | 1 vial | 15 mL |
| Substrate A | 1 vial | 7 mL |
| Substrate B | 1 vial | 7 mL |
| Stop Solution | 1 vial | 7mL |
| Closure Plate Membrane | 2 sheets | |
TEST PROCEDURE
1. All reagents should be allowed to reach 20°C-30°C for 30 minutes before use.
2. Dilute the wash buffer at the rate of 1:20 dilution with distilled water before use.
3. Determine the number of holes required based on the H.P reference material and the specimen to be tested;
4. Add 50μl H.P reference material, controls and 50μl specimen into the corresponding wells;
5. Add 50μl conjugate into each well.
6. Shake gently to mix. Incubate in 37°C for 60 minutes with the sealing plate membrane sealing the plate.
7. At the end of the incubation, remove and discard the plate cover. Take out, add wash buffer to each well. Repeat wash for 5 times. After the final washing cycle, turn the plate over onto blotting paper or clean towel, and tap it to remove any remainders.
8. Add Substrate A (50µL) and Substrate B (50µL), gently shake and mix, and the color was developed at room temperature for 15 minutes;
9. Add 50μl Stop Solution to each well to stop the reaction.
Data processing:
1. Set the microplate reader and read the absorbance of each well using dual wavelength
450nm/630nm.
2. Using a four-parameter fitting method, establish a standard curve and calculate the H.P antigen content of the sample to be teste
