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Address: Add：No.2, Ruitai Rd, Guangzhou Hi-Tech Industrial Development Zone, 510670, CHINA
Contact name:Lisa
Guangzhou BioKey Healthy Technology Co.Ltd |
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Respiratory Syncytial Virus/Influenza B/Influenza A RT-PCR test kit (Lyophilized) --24 tests/kit
Intended use:
This kit is used for in vitro qualitative detection of RSV/Influenza A+B from patients with respiratory tract infection. The test results are for clinical reference only, it cannot be used alone as the basis for the diagnosis or exclusion of cases
Principle:
The kit adopt TaqMan fluorescent probe method. For the highly conserved and specific region of RSV/Influenza A+B, the primer probes, together with RT-PCR reaction solutionfor fluorescent detection are designed and labeled with different fluorescent groups. On the fluorescence quantitative PCR instrument, the real-time fluorescence quantitative PCR detection technology is used to realize the rapid detection of RSV/Influenza A+B through the change of fluorescent signal.
The detection system contains positive internal control, used to monitor whether the nucleic acid extraction process is normal.
Main components:
No. | Components | BIK-QL-H0016S1 |
1 | PCR Enzyme Mix (Lyophilized) | 24 tests/kit |
| 2 | Positive Control | 100 μL/tube |
| 3 | Negative Control | 100 μL/tube |
| 4 | RNase-free water | 1mL/tube |
| 5 | Paraffin oil | 1.5mL/tube |
Note: Do not mix the components from different batches for detection. The Positive Control of RSV/Influenza A+B and internal control were constructed artificially, and they were not infectious.
Specimen requirements:
1.Specimen type :
Throat swab, nasal swab, nasopharyngeal swab, alveolar lavage fluid
and sputum.
2.Specimen preservation:
The specimen can be stored for 3 months at -20±5℃ and for a long
time below -70℃.
Data analysis and interpretation:
The following sample results are possible:
| Ct value | Result Analysis | |
| 1# | No Ct | negative |
| 2# | ≤38 | Positive |
| 3# | 38~40 | Re-test; if it is still 38~40, report as 2# |
Interpretation of test results:
FAM(RSV) | VIC/HEX(Influenza A) | ROX(Influenza B) | CY5(IC) | Interpretation test results | |
1 |
- |
- |
- |
- | Result invalid,test again |
2 |
+ |
- |
- |
+/- | RSV (+) |
| 3 | - | + | - | +/- | Influenza A(+) |
| 4 | - | - | + | +/- | Influenza B(+) |
5 |
+ |
+ |
- |
+/- | RSV ,Influenza A(+) |
6 |
+ |
- |
+ |
+/- | RSV ,Influenza B(+) |
| 7 | - | + | + | +/- | Influenza A+B(+) |
8 |
+ |
+ |
+ |
+/- | RSV ,Influenza A+B(+) |
| 9 | - | - | - | + | Negative(-) |
NOTE: ‘+’ is positive, ‘-’ is negative.
Operation details please check from IFU!
