Home Companies Guangzhou BioKey Healthy Technology Co.Ltd

96 Tests/Kit Trichomonas Vaginalis TV RT PCR Detection Kit Operation Manual

Guangzhou BioKey Healthy Technology Co.Ltd

Contact Us

Trade Verify

Address: Add:No.2, Ruitai Rd, Guangzhou Hi-Tech Industrial Development Zone, 510670, CHINA

Contact name:Lisa

Inquir Now

Guangzhou BioKey Healthy Technology Co.Ltd

Verified Suppliers
  • Trust
    Seal
  • Verified
    Supplier
  • Credit
    Check
  • Capability
    Assessment

96 Tests/Kit Trichomonas Vaginalis TV RT PCR Detection Kit Operation Manual

City & Province guangzhou
InquireNow

Product Details

Trichomonas vaginalis (TV) RT-PCR Detection Kit (Lyophilized) Operation Manual
--96 tests/kit

 
Intended use:
 

By using real time PCR systems, Trichomonas vaginalis (TV) real time PCR kit is used for the in vitro qualitative detection of TV. It can be used for the laboratory diagnosis and monitoring of TV infection. The test results are only for clinical reference, not for case confirmation or exclusion.

 

Principle:
 

The specific primers and specific fluorescent probes are designed for the nucleic acid conserved region of Trichomonas vaginalis (TV). The RT-PCR reaction solution and real-time fluorescent quantitative PCR detection technology is applied on the fluorescence quantitative PCR instrument for realizes the rapid detection of TV through the change of fluorescence signal. The detection system contains a human internal control (IC) gene, which is used to monitor whether the specimens and the process of nucleic acid extraction is qualified.


 
Main components:

 

No.ComponentsBIK-QL-H0020

1

PCR Enzyme Mix(Lyophilized)

96 tests/bottle

2TV Primer-Probe Mix100 μL/vial
3Enzyme Mix Buffer (5×)400 μL/vial
4Positive Control100 μL/tube
5Negative Control100 μL/tube
6RNase-free water1mL/tube
7Paraffin oil1.5mL/tube*2

  
Note: Do not mix the components from different batches for detection. The Positive Control of TV and internal control were constructed artificially, and they were not infectious.

 

Specimen requirements:
 

1. Specimen type

Urine or female vaginal secretions swab samples.

2. Specimen Preservation

The specimen can be stored for 3 months at -20±5℃ and for a long time below -70℃

 

 

PCR Amplification condition:

 
PCR amplification according to the following procedures:
 

StepCycle numberTemperatureTime
1195℃3min
24095℃10s
60℃30s collect fluorescent

 

Instrument detection channel selection: FAM and HEX/VIC.
 
Quality control:
 

 Ct value
Negative ControlNo Ct
Positive Control≤30
IC of samples≤38


Note: Negative Control, Positive Control and IC of samples must be performed correctly, otherwise the sample results is invalid.
 

Data analysis and interpretation:

 

The following sample results are possible:

 Ct valueResult Analysis
1#No Ctnegative
2#≤38Positive
3#38~40Re-test; if it is still 38~40, report as 2#


 Operation details please check from IFU!

Hot Products

Necrotizing Hepato-pancreatitis Bacterium Nucleic Acid Detection Kit (Real time PCR, Lyophilized) ...
Tilapia Lake Virus Nucleic Acid Detection Kit (Real time PCR, Lyophilized) Intended use: This kit is ...
Group B streptococcus(GBS) real-time PCR detection kit (lyophilized) --96 tests/kit Intended use: ...
Group B streptococcus(GBS) real-time PCR detection kit (lyophilized) --24 tests/kit Intended use: ...
Trichomonas vaginalis (TV) RT-PCR Detection Kit (Lyophilized) Operation Manual --24 tests/kit ...
Trichomonas vaginalis (TV) RT-PCR Detection Kit (Lyophilized) Operation Manual --48 tests/kit ...