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Trade Verify
Address: Add：No.2, Ruitai Rd, Guangzhou Hi-Tech Industrial Development Zone, 510670, CHINA
Contact name:Lisa
Guangzhou BioKey Healthy Technology Co.Ltd |
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Verified Suppliers
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Neisseria gonorrhoeae (NG) real-time PCR detection kit (lyophilized)
Intended use:
Principle:
NG product lyophilization pacakage:
Main components:
| No. | Components | BIK-QL-H0023 |
1 | PCR Enzyme Mix (Lyophilized) | 96 tests/bottle |
| 2 | NG Primer-Probe Mix | 100 μL/vial |
| 3 | Enzyme Mix Buffer (5×) | 400 μL/vial |
| 4 | Positive Control | 100 μL/tube |
| 5 | Negative Control | 100 μL/tube |
| 6 | RNase-free water | 1mL/tube |
| 7 | Paraffin oil | 1.5mL/tube*2 |
Specimen requirements:
1. Specimen type
Male urinary tract secretions or female reproductive tract secretions.
2. Specimen Preservation
The specimen can be stored for 3 months at -20±5℃ and for a long time below -70℃
PCR Amplification condition:
PCR amplification according to the following procedures:
| Step | Cycle number | Temperature | Time |
| 1 | 1 | 95℃ | 3min |
| 2 | 40 | 95℃ | 10s |
| 60℃ | 30s collect fluorescent |
Instrument detection channel selection: FAM and HEX/VIC.
Data analysis and interpretation:
The following sample results are possible:
| Ct value | Result Analysis | |
| 1# | No Ct | negative |
| 2# | ≤38 | Positive |
| 3# | 38~40 | Re-test; if it is still 38~40, report as 2# |
Operation details please check from IFU!
