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Agrose Magnetic Beads Protein Purification 30 μm 10% Volume Ratio 100 mL

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Agrose Magnetic Beads Protein Purification 30 μm 10% Volume Ratio 100 mL

Country/Region	china
City & Province	suzhou
Categories	Silver & Sterling Silver Jewelry

Product Details

Magrose Protein G Magbeads for Protein Purification 30-150 μm, 10% (v/v)

1, Description:

BeaverBeads™ Protein A, Protein G are superparamagnetic beads covalently coated with recombinant Protein A or Protein G that contains Fc binding domains for the purification of monoclonal or polyclonal antibodies from cell lysate, blood plasma, ascites and tissue culture supernatant, and for antibody immobilization studies. This product can be used repeatedly, which is suitable for the purification of antibodies in plasma, ascites, tissue culture supernatant and other samples. It can also be used for antibody fixation and other related studies. By referring the attached Table 1 users can select the categories of magnetic beads according to the source and subtype of the target antibody, and compare the affinity of Magrose Protein A and Magrose Protein G beads with different antibodies.

2, Product Information:

Product Name	Magrose Protein A
Bead Size	30~150 μm
Beads Concentration	10% (v/v)
Ligand	Protein A
Medium	Magrose
Antibody Bonding Quantity	25~30 mg Human IgG/mL Gel
Storage Temperature	2~8℃
Binding/Washing Buffer	PBST (pH 7.2~7.4): 137 mM NaCl,2.7 mM KCl, 10 mM Na₂HPO₄, 2.0 mM KH₂PO4, 0.1% Tween-20
Elution buffer	100 mM Gly, 0.1% Tween-20, pH 2.5
Neutrilization buffer	1.0 M Tris-HCl, pH 9.0
Storage buffer	PBST (includes 0.05% NaN3)
Shelf life	12 months

3, Feature:

1. Highly effective antibody binding capacity and ultra-low non-specific adsorption performance

2. Time saving, simple and mild

3. High product stability

4. Antibody elution system is closer to neutral

5. Very low protein A ligand off rate

6. Reusable

4, FAQ:

Q1: How to improve the efficiency of antibody elution?

A1: When the high affinity between antibody and Protein A ligand result in antibody low elution efficiency, users can avoid this situation by reducing the pH value (1.9~2.5) of elution buffer, increasing the ionic strength of elution buffer (2~3 M MgCl2) or prolonging the elution time. But pay attention that antibody could easily aggregate under low pH conditions. Users can adjust the pH to neutral immediately with alkaline buffers (such as Tris, HEPES, etc.).

Q2: How to solve magnetic bead adhere to tube wall?

A2: It is recommended to use the consumables with low adsorption rate. In addition, adding 0.01%~0.1%(v/v) nonionic detergent (such as NP-40, Tween-20 or Triton X-100) to the buffer solution can effectively reduce the adhesion of beads on tube wall.