Recombinant Protein A
No antibody contaminant. No antibody affinity column purification.
E.Coli, HPLC purified.
Protein A mutant of Staphylococcus aureus
Solution：50mg/ml in deionized water or PBS.
PH STABLE RANGE:
-20℃ or below.
The recombinant Protein A is a genetically engineering protein
containing IgG-binding domains. Recombinant Protein A is ideal for
purification of polyclonal or monoclonal IgG antibodies. Protein A
binds to most human and mouse IgG subclasses (e.g., human IgG1,
IgG2, IgG4; mouse IgG2, IgG2a, IgG2b, IgG3). It also binds to cow,
guinea pig, hamster, house, pig and rabbit total IgG form.
Recombinant protein A can be coupled to solid separation medium
(such as agarose) for monoclonal and polyclonal antibody
purification. Recombinant protein A can be coupled to a variety of
molecules (such as fluorescent molecules, enzyme markers, biotin,
colloidal gold and radioactive markers). These coupled derivatives
can be used in antibody test in the process of Western-blot, ELISA
or immunohistochemical tests.
No organisms detected.
Single major band
E270/250≥1.2, E0.1% at 270nm=0.14-0.20
No DNA or RNA detected
No Triton contaminant.
For Research or Manufacturing Purpose Only. Not for Humans.
Figure 1. A.Schematic structure of antibodies B. Structure of SPA
binding to IgG Fc Fragment C. Immobilized SPA binding to IgG
Figure 2. SDS-PAGE of YaxinBio Recombinant Protein A.
1mg 10mg bulk
1mg 10mg bulk