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r-SPA Staphylococcal Recombinant Protein A , fluorescent dyes , enzyme markers , CAS:91932-65-9

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Shanghai Yaxin Biotechnology Co. Ltd

r-SPA Staphylococcal Recombinant Protein A , fluorescent dyes , enzyme markers , CAS:91932-65-9

City & Province Shanghai shanghai
Categories Whey Powder

Product Details

Recombinant Protein A

No antibody contaminant. No antibody affinity column purification.


SOURCE:

E.Coli, HPLC purified.


SEQUENCE:

Protein A mutant of Staphylococcus aureus


MOL. WEIGHT:

25 kDa


FORM:

Lyophilized powder.

Solution:50mg/ml in deionized water or PBS.


PH STABLE RANGE:

pH 1-13


STORAGE CONDITION:

-20℃ or below.


APPLICATION:

The recombinant Protein A is a genetically engineering protein containing IgG-binding domains. Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2, IgG2a, IgG2b, IgG3). It also binds to cow, guinea pig, hamster, house, pig and rabbit total IgG form.

Recombinant protein A can be coupled to solid separation medium (such as agarose) for monoclonal and polyclonal antibody purification. Recombinant protein A can be coupled to a variety of molecules (such as fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers). These coupled derivatives can be used in antibody test in the process of Western-blot, ELISA or immunohistochemical tests.


PRODUCT SPECIFICATION


Test/Method

Specification

Bioburden

No organisms detected.

12% SDS-PAGE

Single major band

Coomassie Stain

25kD

UV Spectrum

E270/250≥1.2, E0.1% at 270nm=0.14-0.20

UV spectrometry

No DNA or RNA detected

Triton Content

No Triton contaminant.


USAGE:

For Research or Manufacturing Purpose Only. Not for Humans.




Figure 1. A.Schematic structure of antibodies B. Structure of SPA binding to IgG Fc Fragment C. Immobilized SPA binding to IgG

Figure 2. SDS-PAGE of YaxinBio Recombinant Protein A.



PRODUCT INFORMATION


Product

Cat.No

Protein Content

Packaging

Manufacturer

Recombinant

Protein A

RSPA05025

≥97%

1mg 10mg bulk

YaxinBio

RSPA05050

≥97%

1mg 10mg bulk

YaxinBio

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