Recombinant trypsin solution , without EDTA and formulated with
human trypsin expressed in E.coli
Recombinant Trypsin Solution
Recombinant trypsin solution, is an animal-component free trypsin
solution optimized for cell dissociation. It is formulated with
recombinant human trypsin / porcine trypsin expressed in E.Coli and refolded and purified by chromatography.
YaxinBio recombinant trypsin is a genetically engineered protein
expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to
porcine pancreas-derived trypsin,with equivalent properties
compared to native trypsin .Recombinant trypsin can replace native
trypsin for using in a variety of biotechnological processes. The
M.W. of recombinant trypsin is 24KD,and the optimum pH is 7.0-11.0.
|Source||Recombinant E. coli|
|Appearance||White or off white, or yellowish powder|
|Specific activity||≥3800 USP units/mg pro.|
|Purity(SDS-PAGE)||Single major band|
|Molecular Weight(SDS-PAGE)||24.0±2.4 kDa|
UNIT DEFINITION:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and
25℃, with BAEE as a substrate (1cm light path).
Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to
aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.
FEATURES AND BENEFITS
Recombinant trypsin,Animal component-free, eliminate the risk of virus and mycoplasma contamination
Recombinant trypsin, no bacteria, mycoplasma, mold and virus was
found and bioburden is conform to biological standard, eliminates
the risk of viruses, or other potential adventitious agents.
Recombinant trypsin owns a similar kinetic of cell detachment to
native trypsin. Soybean trypsin inhibitors and other inhibitors
work the same way with recombinant human trypsin as they do with
Recombinant trypsin provides high specific activity and eliminates
contaminated material found in 1:250 trypsin or other animal origin
The trypsin solution, ready-to-use, eliminates the risk of activity
loss in the process of transport and storage. It also eliminates
the contamination risk that a lyophilized powder has.
Recombinant trypsin solution is formulated at an optimal
concentration to dissociate adherent cells.
Good work without EDTA
No EDTA contained in buffer. And a good cell dissociation result
can be obtained, and then eliminate EDTA influence on cell
apoptosis experimental analysis.