Home Companies Shanghai Yaxin Biotechnology Co.,Ltd.

Recombinant trypsin solution , without EDTA and formulated with human trypsin expressed in E.coli

Contact Us

[China]

Trade Verify

Address: No.466 YinDu Road,XuHui, Shanghai, China

Contact name:zhao

Shanghai Yaxin Biotechnology Co.,Ltd.

Verified Suppliers
  • Trust
    Seal
  • Verified
    Supplier
  • Credit
    Check
  • Capability
    Assessment

Recombinant trypsin solution , without EDTA and formulated with human trypsin expressed in E.coli

Country/Region china
City & Province shanghai shanghai
Categories Flavour and Fragrance

Product Details

Recombinant trypsin solution , without EDTA and formulated with human trypsin expressed in E.coli

 

Recombinant Trypsin Solution

 

DESCRIPTION

Recombinant trypsin solution, is an animal-component free trypsin solution optimized for cell dissociation. It is formulated with recombinant human trypsin / porcine trypsin expressed in E.Coli and refolded and purified by chromatography.

YaxinBio recombinant trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin .Recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes. The M.W. of recombinant trypsin is 24KD,and the optimum pH is pH 7.0-11.0.

 

MAIN FEATURES

SourceRecombinant E. coli
AppearanceWhite or off white, or yellowish powder
Specific activity≥3800 USP units/mg pro.
Purity(SDS-PAGE)Single major band
Molecular Weight(SDS-PAGE)24.0±2.4 kDa

UNIT DEFINITION:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

 

RECOMMEND USAGE

Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.

 

FEATURES AND BENEFITS

Recombinant trypsin,Animal component-free, eliminate the risk of virus and mycoplasma contamination

Recombinant trypsin, no bacteria, mycoplasma, mold and virus was found and bioburden is conform to biological standard, eliminates the risk of viruses, or other potential adventitious agents.

Recombinant trypsin

Recombinant trypsin owns a similar kinetic of cell detachment to native trypsin. Soybean trypsin inhibitors and other inhibitors work the same way with recombinant human trypsin as they do with native trypsin.

High purity

Recombinant trypsin provides high specific activity and eliminates contaminated material found in 1:250 trypsin or other animal origin trypsin.

High safety

The trypsin solution, ready-to-use, eliminates the risk of activity loss in the process of transport and storage. It also eliminates the contamination risk that a lyophilized powder has.

Convenient

Recombinant trypsin solution is formulated at an optimal concentration to dissociate adherent cells.

Good work without EDTA

No EDTA contained in buffer. And a good cell dissociation result can be obtained, and then eliminate EDTA influence on cell apoptosis experimental analysis.

 

 

 

 

Hot Products

Recombinant Trypsin Solution DESCRIPTION Trypsin is a member of the serine protease family. Trypsin ...
Recombinant trypsin solution , without EDTA and formulated with human trypsin expressed in E.coli ...
Recombinant Trypsin Solution DESCRIPTION Recombinant trypsin solution, is an animal-component free ...
Recombinant trypsin solution , the sequence of recombinant human trypsin is same as human trypsin 2 ...
Cell Dissociation solution , Animal component-free , eliminate the risk of virus and mycoplasma ...
Recombinant Trypsin Solution DESCRIPTION Recombinant trypsin solution, is an animal-component free ...