Recombinant protein A which is a membrane protein of Staphylococcus
aureus and expressed in E.coli
Recombinant Protein A
Storage temperature: 2-8℃
Source:Protein A mutant of Staphylococcus aureus , expressed in E. coli
Protein A is a membrane protein of Staphylococcus aureus and can
specifically bind to antibodies. Protein A affinity chromatography
columns have become widely used as affinity columns for purified
antibodies from ascites, serum and cell culture isolated or
purified extracts of antibodies from different subtypes of mammals
or genetically engineered recombinant proteins containing antibody
Fc fragments. alkali protein A is our company independent research
and development production, with independent intellectual property
rights. Recombinant protein A is expressed by E. coli and purified by chromatography to give a purity of 95% or more.
The purification process does not use antibody column affinity
chromatography to avoid the possibility of incorporation of
unrelated IgG in the product.
|Appearance||White or off white powder|
|Bioburden||≤ 1 cfu/ mg|
|SDS-PAGE||Single major band|
|SDS-PAGE (MW)||25000±1000 Daltons|
|UV Spectrum||E275/250 ≥ 1.2|
|E0.1% at 275nm = 0.17-0.23|
|Purity (HPLC)||≥ 95%|
3.STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant protein A lyophilized should be
stored under 2-8℃ in sealed container. It is stable within 24
months.When gluing even with PBS (pH7.4) dissolved,it is now with
the current use.After dissolved wth pure water, it should be stored under -20℃.
Stability of transport: The product is stable by bue ice insulation transport.
1) Non-antibody column purification, the product without any other
antibodies such as IgG and other pollution.
2) SPA-25 is our own research and development products, pH
tolerance range, can withstand 0.5 M NaOH and 0.5M HCl treatment,
no degradation, antibody binding capacity unchanged.
3) The product has a specially designed link for the purification
of the column, can be directly used for the preparation of protein
4) Stable quality: Mass production can ensure stable and continuous
batch production.It is no difference between the batch and the
product quality is stable.
5) High purity: Higher specific activity.Host protein residues is
less than the limits of biological products.
6) Lyophilized powder: The product is lyophilized powder and is
easy to store and transport.
7) Compliance with regulatory requirements: Production equipment
and production environment comply with relevant regulatory
requirements, and the production process is in full compliance with
NSF ISO 9001: 2008 quality system and GMP guidelines.
8) Complete quality documents: we can provide relevant regular
support files in according to customers’ requirement.
The C-terminus of the recombinant protein A contains cysteine which
can be coupled to the solid separation medium for purification of
the monoclonal antibody or polyclonal antibody. Recombinant protein
A binds to most human and mouse lgG subclasses(e.g..human
lgG1,lgG2,lgG4;mouse lgG2,lgG2a,lgG2b,lgG3).It also binds to
cow,guinea pig,hamster,house,pig and rabbbit total lgG
form.Recombinant protein A can be conjugated to a variety of
reporter molecules (fluorescent dyes, enzyme labels, biotin,
colloidal gold, radioactive labels, etc.) without affecting its
binding to the antibody. These conjugated protein A derivatives can
be used for antibody detection of Western-blot, ELISA,
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