Shanghai Yaxin Biotechnology Co.,Ltd. |
protein A , cas number 91932-65-9 , ELISA or Immunohistochemical Tests Antibody Test
CAS:91932-65-9
Source:Protein A mutant of Staphylococcus aureus , expressed in E. Coli.
Application
The recombinant Protein A is a genetically engineering protein containing IgG-binding domains. Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2, IgG2a, IgG2b, IgG3). It also binds to cow, guinea pig, hamster, house, pig and rabbit total IgG form.
Recombinant protein A can be coupled to solid separation medium (such as agarose) for monoclonal and polyclonal antibody purification. Recombinant protein A can be coupled to a variety of molecules (such as fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers). These coupled derivatives can be used in antibody test in the process of Western-blot, ELISA or immunohistochemical tests.
MAIN FEATURES
Appearance | White or off white powder |
Solubility | Clear liquid |
Bioburden | ≤ 1 cfu/ mg |
SDS-PAGE | Single major band |
SDS-PAGE (MW) | 25000±1000 Daltons |
UV Spectrum | E275/250 ≥ 1.2 |
E0.1% at 275nm = 0.17-0.23 | |
Purity (HPLC) | ≥ 95% |