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Product Details

protein A , cas number 91932-65-9 , ELISA or Immunohistochemical Tests Antibody Test

 

Protein A

 

CAS:91932-65-9

Source:Protein A mutant of Staphylococcus aureus , expressed in E. Coli.

 

 

Application

 

The recombinant Protein A is a genetically engineering protein containing IgG-binding domains. Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2, IgG2a, IgG2b, IgG3). It also binds to cow, guinea pig, hamster, house, pig and rabbit total IgG form.

Recombinant protein A can be coupled to solid separation medium (such as agarose) for monoclonal and polyclonal antibody purification. Recombinant protein A can be coupled to a variety of molecules (such as fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers). These coupled derivatives can be used in antibody test in the process of Western-blot, ELISA or immunohistochemical tests.

 

MAIN FEATURES

AppearanceWhite or off white powder
SolubilityClear liquid
Bioburden≤ 1 cfu/ mg
SDS-PAGESingle major band
SDS-PAGE (MW)25000±1000 Daltons
UV SpectrumE275/250 ≥ 1.2
E0.1% at 275nm = 0.17-0.23
Purity (HPLC)≥ 95%