Shanghai Yaxin Biotechnology Co.,Ltd. |
Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification
Recombinant protein A
Application
The recombinant Protein A is a genetically engineering protein containing IgG-binding domains. Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2, IgG2a, IgG2b, IgG3). It also binds to cow, guinea pig, hamster, house, pig and rabbit total IgG form.
Recombinant protein A can be coupled to solid separation medium (such as agarose) for monoclonal and polyclonal antibody purification. Recombinant protein A can be coupled to a variety of molecules (such as fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers). These coupled derivatives can be used in antibody test in the process of Western-blot, ELISA or immunohistochemical tests.
Product Specification
Test/Method | Specification |
Bioburden | No organisms detected. |
12% SDS-PAGE | Single major band |
Coomassie Stain | 25kD |
UV Spectrum | E275/250 ≥1.2, E0.1% at 275nm=0.17-0.23 |
UV spectrometry | No DNA or RNA detected |
Triton Content | No Triton contaminant. |
Format | Lyophilized |
pH stable range | pH 1-13 |
PRODUCT ADVANTAGE
Storage Instructions
Recommend recombinant trypsin lyophilized should be stored under 2-8℃in sealed container.It is stable within 24 months.
Usage
For Research or Manufacturing Purpose Only. Not for Human.