Human Chymotrypsin Proteolytic Enzyme Recombinant Enzyme CAS 9004-07-3 EC 3.4.21.1

Description

Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene sequence is the same as human chymotrypsin. Recombinant chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in pH 7.0–9.0.

Advantages

Animal origin free:Recombinant human chymotrypsin eliminates the risk of virus presence, and other potential adventitious agents found in animal-derived chymotrypsin.

High purity:Recombinant Chymotrypsin provides increased specificity and eliminates contaminated other proteases activities found in enzymes purified from pancreas.

Stable:A sterile recombinant human chymotrypsin lyophilized decreases the contamination risks and chance of activity loss during transport and storage.       

RECOMMEND USAGE

Prepare 1-10mg/ml recombinant human chymotrypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-9.0.

STABILITY OF STORAGE AND TRANSPORT

Stability of storage: Recombinant human chymotrypsin lyophilized should be stored under 2-8℃ in sealed container.

It is stable within 24 months.After dissolved with 1mM HCl , it should be stored under -20℃. Avoid repeated freezing and thawing.

Stability of transport: The product is stable by blue ice insulation transport.

Application

Hydrolysis of proteins by chymotrypsin alone or in combination with other proteases. Suitable for peptide mapping, fingerprinting, and sequence analysis.