Viral RNA&DNA Extraction Kit 48 tests for obtaining purified
Nucleic Acid (Magnetic Bead Method)
【PRODUCT NAME】
Viral RNA&DNA Extraction Kit
【METHOD】
Magnetic Bead Method
【PACKAGE SPECIFICATION】
48 tests/ box
【INTENDED USE】
This kit is used to extract DNA/RNA from plasma, serum, animal
tissues, body fluids
and other samples. The optimized chemistry of the reagents ensures pure
nucleic
acid is selectively bound to the paramagnetic bead particles, and
impurities are
efficiently removed by a series of quick wash steps. The resulting high-quality
DNA/RNA is eluted with elution buffer or water. The Viral RNA&DNA Extraction Kit
can be easily adapted to automated magnetic bead separation instruments and
workstations. The nucleic acid extracted by this kit can be used for Re a-time PCR,
Nothern Blot, Blotting Hybridization, cDNA Library Construction.
【COMPONENTS OF THE KIT】
| Components | 96T/Kit |
| Magnetic Rod Sleeve | 1 piece/kit |
| Prefilled 96Deep Well Plate (Lysis Solution) | 96T×1 plate |
| Prefilled 96Deep Well Plate (Magnetic Beads) | 96T×1 plate |
| Prefilled 96Deep Well Plate (Wash Buffer 1) | 96T×1 plate |
| Prefilled 96Deep Well Plate (Wash Buffer 2) | 96T×1 plate |
| Prefilled 96Deep Well Plate (Elution Solution) | 96T×1 plate |
【STORAGEAND STABILITY】
The kit should be stored at room temperature 5-25℃. The kit is
valid for 12 months.
【APPLICABLE INSTRUMENTS】
It is suitable for the automatic nucleic acid extraction instrument
such as RJ40.
【SPECIMEN REQUIREMENTS】
1.The virus in liquid samples such as plasma, serum, ascites, oral
fluid can be directly
extracted.
2.The virus in animal and plant tissues needs to add an appropriate
amount of saline or
PBS to the sample, grind it thoroughly, centrifuge at 12000rpm for
5-10 minutes, and
take the supernatant for use.
【SPECIMEN EXTRATION OPERATION】
1.Take out the pre-packed deep well plate, tear off the sealing
film, and add 200ul
of the above serum, plasma, throat swab, nasal swab, saliva, and other
samples to
the Lysis Solution plate hole. In accordance with the order of the plate
positions
(1, 2, 3, 4, 6), put the Lysis Solution Plate (1), Magnetic Beads Plate (2), Wash Buffer
l
Plate (3), Wash Buffer 2 Plate(4) and the Elution Solution Plate(6) in the
automatic
nucleic acid extractor, and install the Magnetic Rod Sleeve.
2.Turn on the nucleic acid extractor, set the following program and
run it(-15min);
| NO. | Step | Site (1-6) | Volume (µl) | Mixing | Delay | Binding | Bind Height (mm) | Heating |
| Time (min) | Speed (1-10) | Time (min) | Position | Move (min) | Top (sec) | Bottom (sec) | Site (1/6) | Temp (ºC) |
| 1 | Move | 2 | 250 | 0.1 | 8 | 0 | - | 0.5 | 10 | 10 | Auto | Off | - |
| 2 | Lysis | 1 | 800 | 4.0 | 8 | 0 | - | 0.5 | 10 | 10 | Auto | 1 | 65 |
| 3 | Wash1 | 3 | 600 | 1.0 | 8 | 0 | - | 0.5 | 10 | 10 | Auto | Off | - |
| 4 | Wash2 | 4 | 600 | 1.0 | 8 | 0 | - | 0.5 | 10 | 10 | Auto | Off | - |
| 5 | Elution | 6 | 80 | 2.0 | 8 | 0 | - | 0.5 | 10 | 10 | Auto | 6 | 85 |
| 6 | Drop | 2 | 250 | 0.3 | 8 | 0 | - | 0 | 0 | | Auto | Off | - |
3. At the end of the run remove the magnetic rod sleeves；
4. Take out the 96 deep well plate, transfer the purified DNA/RNA
into a 1.5ml
centrifuge tube from the plate with Elution Solution in order for
the following
operation or store at-80℃ (If there is a small number of magnetic beads
remaining,
it can be removed by centrifugation or magnetic plate);
5.Close the program and shutdown the instrument.
【PRODUCT PERFORMANCE INDEX】
1. The purity:The ratio of absorbance OD260/OD280 is between
1.8-2.2.
2. The precision:The coefficient of variation (CV) of the RNA/DNA
content is≤5%.
【PRECAUTIONS】
1.Please read this kit's operating instructions carefully before
use, and operate
carefully as required.
2.The virus has a strong infection ability, and various defense
measures must be
prepared before operation.
3.Wear clean gloves and masks during operation.
4.Use RNase/DNase-free tips and 1.5ml centrifuge tubes.
5.All centrifugation steps are performed at room temperature
(5-25°℃) .
6.Frozen samples need to be thawed to room temperature.
7.Do not use products beyond the expiration date; do not mix
reagents of different
lot numbers.
【INDEX OF SYMBOL】
【EXPORTER】
Magnus International Limited
F12, New City International Mansion A, 234 Huapao Ave.
Liuyang, Hunan Province 410300 China
Contact: Goodwellmedical@gmail.com
【AUTHORIZED REPRESENTATIVE】
Lotus NL B.V.
Koningin Julianaplein 10, 1e Verd, 2595AA, The Hague, Netherlands.
【FACTORY VIEW】
【FACTORY WORKSHOP】
【PRODUCT AND STANDARDS】
【CERTIFICATIONS】
【FAQ】
1. Q: How about your healthcare and medical product quality?
A: Quality is our top priority. We have a professional quality
control team to ensure the
quality with a high standard and regulated with the pro
certifications. The QC team is
responsible for quality checking in each process and inspects every
batch of our production
and ships them only after QC approval.
2. Q: What is your order MOQ?
A: Please contact customer service, and the price is negotiable for
a large quantity.
3. Q: How about your product packing for shipment or mailing?
A: We prepared a well neutral package for our products. We could
also make OEM packages,
OEM/ODM Custom Package/Multi-packing package/Cartons with well
protective wrapping.
4. Q: What level of your product price?
A: We are the factory and direct manufacturer and offer competitive
prices. We also offer
volume pricing with more benefits depending on your order quantity
level. Please let us know
your ordering volume while you make the inquiry to us to help you
obtain better benefits.
5. Q: What're the price terms?
A: The price is based on the FOB of Hunan, China with an exchange
rate of RMB6.40 for
USD$1.00. If the exchange rate floating sharply lower than 6.3 or higher than
6.5, we should
adjust the price with the real-time exchange rate.
6. Q: What're the payment terms?
A: We accept T/T wire transferring, PayPal, Western Union, etc. 50%
down payment after
signing the contract and rest 50% before shipping confirmation.
7. Q: How could I get some samples?
A: We are able to deduct your samples expense once your orders are
confirmed with us.
The shipping fee will be calculated with the sample volume that you
demand.
8. Q: Could you accept OEM orders?
A: Yes, we could. We have professional designers who can design the
logo or artwork and offer
best solution & styles for your OEM product choices.
9. Q: How long will the shipping or express take?
A: (1) For the international express such as DHL, UPS, FedEx, TNT,
or EMS, etc., it usually
takes 5-7 working days to your location or destination.
(2) If shipping with containers, they will take about 30-60 days to
process depending on
the various ports or door locations.
(3) We will arrange the delivery or shipping of your products
within 1-3 days after full
payment has been confirmed. We may not ship your products out until your
payments arrive
to our bank account successfully.
10. Q: How do you deliver the goods to us?
A: If the order of small volume package, we could deliver by
courier such as DHL, UPS, FedEx,
TNT, EMS. For the large volume order, we could ship with bulk cargo
containers by sea.
We will provide you with cost-saving recommendations for your
product shipping.
11. Q: What is the warranty time.
A: One year for each product.
【SHIPPING】
We are the trusted healthcare and medical supplies from China.
Be free to contact us at any time!
You are very welcome to email us today at:
Goodwellmedical@gmail.com for the inquiry!
We are excited to build a reliable and long-term business with you
at our best!
