CH2COOH-PEG5-CH2COOH With Cas.77855-76-6 Is For Surface Or Particle Modifications.

Introduction

Typical PEGylation reagents contain heterogeneous mixtures of different PEG chain lengths; however, our PEGylation reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimizing modification applications.

The CH2COOH-PEG5-CH2COOH With Cas. 77855-76-6 is used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers. Modification of proteins adds polyethylene glycol (PEG)_n spacers, which impart increased water solubility, reduced immunogenicity of the labeled molecule and enhanced in vivo stability in solution. Functionalization of solid surfaces, such as quantum dots, self-assembled monolayers and nanoparticles, with polyethylene glycol spacers significantly reduces nonspecific protein binding. (PEG)n amine reagents used with (PEG)n amine reagents in surface modification can form a hydrophilic “lawn” of methyl ether-terminated PEGs with periodic exposed carboxy-terminated PEGs. The exposed carboxy groups can be coupled to affinity ligands using the carbodiimide coupling reaction with EDC and sulfo-NHS.

Important Product Information

• The CH2COOH-PEG5-CH2COOH With Cas.77855-76-6 is low-melting solids that are difficult to weigh and dispense. To facilitate handling, make a stock solution by dissolving the reagent with dimethylsulfoxide (DMSO) or dimethylformamide (DMF).

• Use the (PEG)n amine reagents in combination with (PEG)n amine reagents to modify surfaces and minimize nonspecific binding.

• Use non - amine - containing buffers at pH 7-9 such as PBS (20mM sodium phosphate, 150mM NaCl; pH 7.4); 20mM HEPES; 100mM carbonate/biocarbonate; or 50mM borate. Do not use buffers that contain primary amines, such as Tris or glycine, which compete with acylation.

Tags: Methyl pegs, acid pegs, COOH pegs, NHS pegs