Amino-PEG8-T-Butyl ester With Cas.756526-06-4 Is For Chemical Modifications

Synonyms:

H2N - PEG8 - tBu ; 27 - Amino - 4 , 7 , 10 , 13 , 16 , 19 , 22 , 25 - octaoxaheptacosanoic acid 1 , 1 -dimethylethyl ester ; H2N - PEG8 - CH2CH2COOtBu ; Amino - PEG8 - t - butyl ester ; Amino - PEG9 - t - butyl ester ; H₂N - PEG₈ - CH₂CH₂COO^tBu.

Introduction

The Amino-PEG8-T-Butyl ester With Cas.756526-06-4 is used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers. Modification of proteins adds polyethylene glycol (PEG)_n spacers, which impart increased water solubility, reduced immunogenicity of the labeled molecule and enhanced in vivo stability in solution. Functionalization of solid surfaces, such as quantum dots, self-assembled monolayers and nanoparticles, with polyethylene glycol spacers significantly reduces nonspecific protein binding. (PEG)n amine reagents used with (PEG)n amine reagents in surface modification can form a hydrophilic “lawn” of methyl ether-terminated PEGs with periodic exposed carboxy-terminated PEGs. The exposed carboxy groups can be coupled to affinity ligands using the carbodiimide coupling reaction with EDC and sulfo-NHS.

Typical PEGylation reagents contain heterogeneous mixtures of different PEG chain lengths; however, our PEGylation reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimizing modification applications.

Important Product Information

• The Amino-PEG8-T-Butyl ester With Cas.756526-06-4 is low-melting solids that are difficult to weigh and dispense. To facilitate handling, make a stock solution by dissolving the reagent with dimethylsulfoxide (DMSO) or dimethylformamide (DMF).

• Use the (PEG)n amine reagents in combination with (PEG)n amine reagents to modify surfaces and minimize nonspecific binding.

• Use non-amine-containing buffers at pH 7-9 such as PBS (20mM sodium phosphate, 150mM NaCl; pH 7.4); 20mM HEPES; 100mM carbonate/biocarbonate; or 50mM borate. Do not use buffers that contain primary amines, such as Tris or glycine, which compete with acylation.