Amino-PEG2-Azide Is A Kind Of PEG Contaning An Amine Group

Introduction

The Amino-PEG2-Azide is used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers. PEG Azides, a Polyethylene Glycol Building Blocks for PEGylation. It contaians a free amine group and an azido group, which The azide group enables Click Chemistry. Modification of proteins adds polyethylene glycol (PEG)_n spacers, which impart increased water solubility, reduced immunogenicity of the labeled molecule and enhanced in vivo stability in solution. Functionalization of solid surfaces, such as quantum dots, self-assembled monolayers and nanoparticles, with polyethylene glycol spacers significantly reduces nonspecific protein binding. (PEG)n amine reagents used with (PEG)n amine reagents in surface modification can form a hydrophilic “lawn” of methyl ether-terminated PEGs with periodic exposed carboxy-terminated PEGs. The exposed carboxy groups can be coupled to affinity ligands using the carbodiimide coupling reaction with EDC and sulfo-NHS.

Typical PEGylation reagents contain heterogeneous mixtures of different PEG chain lengths; however, our PEGylation reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimizing modification applications.

Amine-containing PEG linkers are widely used to modify water-soluble biopolymers (such as proteins) using water-soluble carbodiimides (such as EDC) to convert the carboxyl groups of the biopolymers into amide groups. Either NHS or NHSS may be used to improve the coupling efficiency of EDC-mediated protein– carboxylic acid conjugations. A large excess of the amine-PEG containing dyes is usually used for EDCmediated bioconjugations in concentrated protein solutions at low pH to reduce intra- and inter-protein coupling to lysine residues, a common side reaction. These dyes can also be used for modifications of carbohydrates, glycoproteins, and nucleic acids that are first periodate-oxidized to introduce aldehydes ketones into the biopolymers for subsequent reductive amination. The combination of periodate oxidation with reductive amination provides an effective way for site-selective modifications of biopolymers. For example, periodate oxidation of the 3’-terminal ribose is reported to be one of the few methods of selectively modifying RNA. Periodate-oxidized ribonucleotides are converted to fluorescent nucleotide probes by reaction with fluorescent-PEG hydrazines and amines.

Important Product Information

• The Amino-PEG2-Azide is low-melting solids that are difficult to weigh and dispense. To facilitate handling, make a stock solution by dissolving the reagent with dimethylsulfoxide (DMSO) or dimethylformamide (DMF).

• Use the (PEG)n amine reagents in combination with (PEG)n amine reagents to modify surfaces and minimize nonspecific binding.

• Use non-amine-containing buffers at pH 7-9 such as PBS (20mM sodium phosphate, 150mM NaCl; pH 7.4); 20mM HEPES; 100mM carbonate/biocarbonate; or 50mM borate. Do not use buffers that contain primary amines, such as Tris or glycine, which compete with acylation.