Home Companies Shanghai Upper Bio-Tech Pharma Co.,Ltd

CRP-DOT

Shanghai Upper Bio-Tech Pharma Co.,Ltd

CRP-DOT

Country/Region China
Categories Massage Hammer

Product Details

CRP-DOT

Rapid Quantitative Determination for CRP
(C-Reactive Protein) by DIGFA Method
1.Product description
1.1 Intended Use
CRP-DOT is an in vitro test for the rapid determination of CRP(C-Reactive Protein) in human serum, plasma and whole blood.
1.2 Summary
The detection of CRP in serum, plasma and whole blood is an important indicator for the distinction between bacterial and virus infections. C-Reactive Protein is in an acute phase and it's concentration in normal human blood is low. CRP levels rise rapidly when there is bacterial infection, and it will drop back to normal level after recovery.
1.3 test principle
CRP-DOT is a solid phase, sandwich-format, immunometric assay. The test device contains membrane coated with immobilized CRP specific monoclonal antibodies, When the serum or urine sample is applied to the test device, the sample flows through the membrane, and immobilized antibodies on the membrane capture the CRP molecules. CRP trapped on the membrane will bind the gold-antibody conjugate then added, in a sandwich-type reaction. Unbound conjugate is removed from the membrane by the washing solution. The paper layer underneath the membrane absorbs excess liquid. In the presence of a pathological level of CRP in the sample, the membrane appears purple with colour intensity proportional to CRP concentration of the sample. The colour intensity is measured quantitatively by using the colour densitometer UpperGold U2 Reader.
1.4 Kit contents, 20 test kit
Test Device
1×20 units
Plastic device containing a membrane coated with monoclonal anti-CRP antibodies.
Dilution Liquid
1×20 units
Glycin-NaOH buffer (PH7.6).
Lyophilized Gold-Conjugate
2 ×1.1 mL
Lyophilized conjugate containing monoclonal anti-CRP antibodies labelled with ultra-small gold particles.
Regeneration Solution
13.0 mL
0.66% NaCl solution.
Blocking Solution
13.0 mL
Phosphate buffered NaCl solution.
Washing Solution
17.0 mL
Phosphate buffered NaCl solution.
1.5 Materials required but not provided with the kit
1. Pipette and pipette tips for dilution of sample and application of diluted sample and regeneration of Lyophilized Gold-Conjugate.
2. UpperGold U2 Reader for measurement of the test result.
1.6 Warning and precautions
1. For in vitro diagnostic use only.
2. Dilution Liquid, Lyophilized Gold-Conjugate and Blocking Solution contains sodium azide (1mg/ml), a toxic agent.
2.Product properties

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