CRP-DOT is a solid phase, sandwich-format, immunometric assay. The
test device contains membrane coated with immobilized CRP specific
monoclonal antibodies, When the serum or urine sample is applied to
the test device, the sample flows through the membrane, and
immobilized antibodies on the membrane capture the CRP molecules.
CRP trapped on the membrane will bind the gold-antibody conjugate
then added, in a sandwich-type reaction. Unbound conjugate is
removed from the membrane by the washing solution. The paper layer
underneath the membrane absorbs excess liquid. In the presence of a
pathological level of CRP in the sample, the membrane appears
purple with colour intensity proportional to CRP concentration of
the sample. The colour intensity is measured quantitatively by
using the colour densitometer UpperGold U2 Reader.
1.4 Kit contents, 20 test kit
Plastic device containing a membrane coated with monoclonal
anti-CRP antibodies.
Dilution Liquid | 1×20 units |
Glycin-NaOH buffer (PH7.6).
Lyophilized Gold-Conjugate | 2 ×1.1 mL |
Lyophilized conjugate containing monoclonal anti-CRP antibodies
labelled with ultra-small gold particles.
Regeneration Solution | 13.0 mL |
0.66% NaCl solution.
Phosphate buffered NaCl solution.
Phosphate buffered NaCl solution.
1.5 Materials required but not provided with the kit
1. Pipette and pipette tips for dilution of sample and application
of diluted sample and regeneration of Lyophilized Gold-Conjugate.
2. UpperGold U2 Reader for measurement of the test result.
1.6 Warning and precautions
1. For in vitro diagnostic use only.
2. Dilution Liquid, Lyophilized Gold-Conjugate and Blocking
Solution contains sodium azide (1mg/ml), a toxic agent.
2.Product properties