High Capacity Wide Range Of Applications Automated Nucleic Acid
Extractor is based on the magnetic bead method to extract DNA in
the sample. It uses specially designed nanomagnetic beads to absorb
DNA under different conditions. By adsorbing, washing and eluting
the DNA in the sample Wait for a series of operation procedures to
obtain purified DNA template. The main extraction process is
divided into the following three steps:
(1) Sample processing: processing according to different
sample types, cutting or drawing a small amount of sample, and
adding it to a 1.5ml Ep tube;
(2) Lysis release: Add the lysis solution to the
above-treated sample, mix it evenly (vortex and shake), and then
place it in a constant temperature incubator at 65°C for 30-60
minutes;
(3) Separation and purification: add the incubated sample to
the single sample or whole plate extraction kit of the instrument,
and run the edited automatic extraction program to obtain the
purified DNA template.
Among them, the single-sample extraction kit can extract any 1~8
samples, the whole-plate extraction kit can extract 16 samples, and
a maximum of 32 samples can be extracted in one run (two plates),
providing a flexible and convenient extraction scheme and saving
reagent consumables costs . The instrument uses the cooperation of
the magnetic rod and the magnetic rod cover to achieve the
adsorption and release of the magnetic beads and complete the
sample DNA extraction process.
Nucleic Acid Extractor Parameters
| Model | AU1001-96 |
| Brand Name | HAIYING |
| Instrument classification | Class I |
| After-sales Service Provided | Online support |
| magnetic head | 96 |
Nucleic Acid Extractor Application
High Capacity Wide Range Of Applications Automated Nucleic Acid
Extractor is generally divided into two types: suction method and
magnetic rod method:
1. The suction method, also called the pipetting method, is
to extract nucleic acid by fixing magnetic beads and transferring
liquid. Generally, the transfer is realized by controlling the
mechanical arm through the operating system. The extraction process
is as follows:
1) Lysis: Add the lysis solution to the sample, realize the
mixing and full reaction of the reaction solution through
mechanical movement and heating, cell lysis, and release of nucleic
acid.
2) Adsorption: Add magnetic beads to the sample lysing
solution and mix them thoroughly. The magnetic beads have a strong
affinity for nucleic acids under high salt and low pH values to
adsorb nucleic acids. Under the action of an external magnetic
field, the magnetic beads are separated from the solution. , Use
the tip to remove the liquid and discard it to the waste tank, and
discard the tip.
3) Washing: Remove the external magnetic field, replace with
a new tip, add washing buffer solution, mix thoroughly to remove
impurities, and remove the liquid under the action of an external
magnetic field.
4) Elution: Remove the external magnetic field, replace with
a new tip, add elution buffer, mix thoroughly, and the bound
nucleic acid is separated from the magnetic beads to obtain
purified nucleic acid.
2. The magnetic rod method realizes the separation of nucleic
acids by fixing the liquid and transferring the magnetic beads. The
principle and process are the same as the suction method, but the
difference is the method of separating the magnetic beads and the
liquid. The magnetic rod method is to separate the magnetic beads
from the waste liquid through the adsorption of the magnetic beads
on the magnetic rod, and put them into the next liquid to realize
the extraction of nucleic acid.
