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Sperm DNA Fragmentation Sperm Chromatin Structure Assay Kit AOT
Flow Cell Methodology
Packing specification:
Intended use:
Test principle:
After denatured by acid, fragmented sperm chromatin would turn to single strand. And the normal sperm chromatin retain double strand. Acridine Orange(AO) associated with single-stranded DNA emits red or yellow fluorescence at 488nm luminescence. While AO intercalated in double stranded DNA emits green fluorescene. If the red fluorescence is increasing, it shows a high rate of DNA fragmentation in sperms.
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Specimen requirements: