Shenzhen Lvshiyuan Biotechnology Co.,Ltd |
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Tetracyclines rapid test dipstick (Eggs)
For sample milk, tissue, honey is available
Catalog No. LSY-20046
1. Brief
This product is used for testing Tetracyclines residue in egg sample qualitatively. The total test time need 5-10 min. Easy to operate, high sensitivity.
2. Detection limit
50 ng/ml (50 ppb)
3. Specifications: 8 dipsticks/bottle, 12 bottles/box
4. Principle
The Tetracyclines rapid test dipstick is based on competitive inhibition immuno-chromatographic principle. In the flow process, Tetracyclines in the sample combined with Tetracyclines specific colloidal gold-labeled monoclonal antibody, Inhibit the combination between antibody and Tetracyclines -BSA conjugate on Test line of NC membrane, lead to the color change of Test line.
When the sample has no Tetracyclines residue or concentration lower than detection limit, T line appears; when the concentration is equal to or higher than detection limit, T line has no color. No matter whether there is Tetracyclines residue in sample, C line will appear, it means the test is valid.
5. Contents
1 | Tetracyclines test dipsticks | 8 dipsticks/bottle, 12 bottles |
2 | Red powder micro-wells | 96 wells |
3 | Manual | 1 piece |
4 | Desiccant | 2 pieces/bottle |
6. Sample preparation
Weigh 1g fresh mix evenly egg sample, add 3ml pure water, shake strongly for 3min, ready to test.
7.Operation procedures
7.1 Read the instruction carefully before use. Return test dipsticks and sample into room temperature.
7.2 Take bottles needed from the kit package, take out required micro-wells and dipsticks, making proper marks. Please use these test dipsticks within 1h. Seal the cap of the bottles, avoid moisture.
7.3 Take 200ul of the test samples into the micro-wells, then repeatedly absorb for 5 times, mix the sample with the reagent in themicro-wells completely until no solid judge by eyes (this is a very important step).
7.4 Incubate for 5min at room temperature (20-25℃), then insert the test dipsticks into the micro-wells with the "MAX" end fully dipped in to the mixture solution.
7.5 Incubate for 5-8min at room temperature again, read the result, it is invalid in other time.
8. Test Result Interpretation
8.1 Negative: C line appears, T line is darker than C line, or T line has same color with C line. It means there is noTetracyclines residue in sample or the residue is lower than detection limit.
8.2 Positive: C line appears, T line is obviously lighter than C line or T line is invisible. It means the Tetracyclines residue is equal to or higher than detection limit.
8.3 Invalidation: C line isn’t seen wine red. It means the test card is out of efficacy, out of date or improper operation. Please run the test again using another package. If the invalid tests keep happening, please contact the supplier.
Negative Negative Positive Invalid Invalid
9.Specificity
This product has no cross reaction with Quinolones, Streptomycin etc.
10. Precautions
1) The test strips can be used only once at room temperature, do not use strips out of expiry date.
2) Every tips is single use only, to avoid cross pollution.
3) Do not touch the white membrane surface in the middle of test strip, avoid sunlight and fan blowing directly.
4) Tap water, distilled water and deionized water can not treat as negative control.
5) Please contact the supplier for any questions.
11. Storage and expiry date
Storage: Store at 2-8 ℃ in dark, sealed, dry place, no frozen.
Expiry date: 12 months; date of production is on box.