Active Member
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[China]
Address: D building, National Biological Industrial Park of Marinelife, No.2 Binghai Road, Dapeng, Shenzhen, China 518120
Contact name:Bella Zou
Shenzhen Lvshiyuan Biotechnology Co.,Ltd |
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| Brand: Green Spring |
| Swine Foot and Mouth Disease (FMD) IgG Distinguishing ELISA kit Catalogue No.:LSY-30003 |
| Brief Foot-and-mouth disease (FMD) is a severe, highly communicable viral
disease of cattle and swine. It also affects sheep, goats, deer,
and other cloven-hoofed ruminants. FMD is not recognized as a
zoonotic disease. The disease is characterized by fever and blister-like lesions
followed by erosions on the tongue and lips, in the mouth, on the
teats, and between the hooves. Many affected animals recover, but
the disease leaves them debilitated. It causes severe losses in the
production of meat and milk. Because it spreads widely and rapidly and because it has grave
economic as well as clinical consequences, FMD is one of the animal
diseases that livestock owners dread most. The GreenSpringSwine Foot and Mouth Disease (FMD) IgG Distinguishing ELISA Test
kit is used for detection the non-structural 3ABC-protein of swine
foot and mouth disease (FMD-3ABC) antibodies in the serum,
distinguishment naturally infected virus from vaccined swine. |
| Principle The GreenSpring Swine Foot and Mouth Disease (FMD) IgG
Distinguishing ELISA Test kit is the antigens coated microplate
made through FMD-3ABC protein. In the test, the diluted control
serum and sample are added, then incubate. If FMD-3ABC specific
antibodies exist in the sample, it will be bound with the antigens
of the microplate but the unbound antibodies and other components
will be removed by washing. Next add enzyme conjugate (anti-pig
IgG-HRP) to specifically bind with the compound of antibodies and
antigens on the microplate. Then the unbound conjugates will be
removed by washing, Add the TMB substrate in the well, react with
HRP to a blue product. At last, end the reaction by adding stop
solution. |
| Specifications Reagents of the Test Kit 1 Antigen coated microplate 2 pieces (96 wells) 2 Negative control serum(green lid) 1 tube (1ml/tube) Positive control serum(red lid)............................. 1 tube
(1ml/tube) 3 Anti-pig IgG-HRP combination(yellow lid).......... .1 bottle
(22ml/bottle) 4 20× concentrated washing solution (white lid)... 1 bottle
(30ml/bottle) 5 Substrate A (red lid)/B (black lid)............. 1 bottle each
(12ml/bottle) 6 Stop solution (blue lid).................................. 1
bottle (12ml/bottle) 7 Sample diluent solution (transparent lid)........... 1 bottle
(50ml/bottle) Storage:store 2-8℃,not frozen. Expiry date:6 months |
