Active Member
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[China]
Address: D building, National Biological Industrial Park of Marinelife, No.2 Binghai Road, Dapeng, Shenzhen, China 518120
Contact name:Bella Zou
Shenzhen Lvshiyuan Biotechnology Co.,Ltd |
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| Brand: Green Spring |
| PRRS RT-PCR test kit |
| Brief This test was assessed for sensitivity and application as a rapid
diagnostic tool by comparison with virus isolation and detection of
PRRS virus antibody in blood. The RT-PCR test was slightly more
sensitive than virus isolation for detection of virus in serum and
markedly more sensitive than virus isolation from plasma from
experimentally infected pigs. |
| Principle RNA in refrigerated serum sample is extracted by TRIzol , The cDNA
synthesis was performed with RNA template and specific primer . To
produce fragment of PRRSV , degenerate at high temperature, anneal
at low temperature, transcript at mid-temperature, cycle with Taq
DNA , amplify million fold through 35 times cycle. Electrophoresize
RT-PCR products , dyed by ETBR, generate specific fragments in the
ultraviolet lights which can be seen by eyes. |
| Specifications Components: A. solution: Positive control 60 ul B. solution: TriZol solution 7ml C. solution: CHCl33.5ml D. solution: C3H3O 4ml E. solution: CH3CH2OH 12ml F. solution: DEPC-Deionized water: 500ul G. solution:RT-PCR buffer 22.5ul X11 H. solution:sample buffer: 50ul Storage:B, C,D,H, stored at 4-8 ℃; A,E,F,G, stored at -20 ℃;don’t froze
repeatedly. Expiry date:3 months. Specification:10 test |
