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Taq DNA Polymerase
#P1011, 500U
Concentration: 5U/μl
Contents:
| Taq DNA Polymerase | 100 μl |
| 10× PCR Buffer (Mg2+ Free) | 1.25 ml |
| 6× Loading Buffer | 1 ml |
| 25mM MgCl2 | 1.25 ml |
Store at -20°C
For research use only.
In total 4 vials.
Description
Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5 kb (simple template). The elongation velocity is 2kb/min (70~75°C). It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that results in a 3'-dA overhangs PCR product.
Unit Definition
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.
Storage Buffer
20mM Tris-HCl (pH 8.0), 100mM KCl, 3.2mM MgCl2, 1mM DTT, 0.1% Triton X-100, 0.1% Tween20, 0.2mg/ml BSA, 50% (V/V) glycerol.
10× PCR Buffer without Mg2+
100mM Tris-HCl (pH 8.8), 500mM KCl, 1% Triton X-100.
Applications
• PCR amplification of DNA fragments as long as 5 kb
• DNA labeling
• DNA sequencing
• PCR for cloning
GDSBio has a complete product line. With PCR technology as the core, focusing on molecular biology techniques such as ordinary PCR, fluorescence quantitative PCR, NGS library preparation, nucleic acid electrophoresis, nucleic acid purification, etc.It has developed reagents for molecular biology research and diagnosis, and completed the layout of the whole industrial chain from molecular biology reagents, medical lab supplies to specialized services.
Product operation manual:P1011(mg2+free)Taq DNA Polymerase V1.2.pdf
