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QPCR Hotstart Taq DNA Polymerase
For research use only
Components
| Component | P1101 250 U | P1102 1,000 U | P1103 3,000 U | P1104 18,000 U |
| qPCR Hotstart Taq DNA Polymerase (5U/ul) | 50 μl | 200 μl | 200 μl × 3 | 100 μl × 36 |
| 10× qPCR Buffer[1] | 1.25 ml | 1.25 ml × 2 | 1.25 ml × 6 | 1.25 ml × 36 |
[1] qPCR Buffer contains Mg2+ and other components that are necessary for qPCR.
Storage
This reagent should be kept at -20°C. The shelf life is 2 years when stored properly.
Description
QPCR Hotstart Taq DNA Polymerase is a hot-start Taq DNA polymerase modified by antibody-like technology, and the activity is wholly inhibited at room temperature, which results in less non-specific amplifications. Depending on high temperature to activate the enzyme activity, the reaction has very high specificity and sensitivity. QPCR Hotstart Taq DNA Polymerase adopts advanced production technology, does not introduce contamination of animal-derived DNA. The enzyme has stronger stability, and incomparable advantages over antibody-modified hot-start Taq DNA polymerase. The pre-denaturation time is reduced to 3 minutes, and the amplification efficiency is higher than that of most chemical-modified hot-start DNA polymerase. The elongation rate is 1kb/min. It is a special polymerase for real-time quantitative PCR (qPCR), both dye method and probe method.
Unit Definition
Using activated salmon sperm DNA as template/primer, the activity of ingesting 10 nmol total nucleotides as acid insoluble substances within 30 min at 74°C is defined as 1 unit of activity (U).
Storage Buffer
200mM Tris-HCl, 1mM DTT, 0.1mM EDTA, 100mM KCl, 0.5% Tween20, 50% (V/V) glycerol, 0.5% Triton X -100
10× qPCR Buffer with Mg2+
50mM KCl, 100mM Tris-HCl, 200mM NH4Cl, 20mM MgCl2
Applications
High-specificity amplification & multiplex qPCR: hot-start polymerase with antibody-like modification
Thermostable: half-life over 40 min at 95°C incubation
Generates 3'-dA overhangs PCR products
GDSBio has a complete product line. With PCR technology as the core, focusing on molecular biology techniques such as ordinary PCR, fluorescence quantitative PCR, nucleic acid electrophoresis, nucleic acid purification, it has developed reagents for molecular biology research and diagnosis, and completed the layout of the whole industrial chain from molecular biology reagents, medical lab supplies to specialized services.
