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Long Taq DNA Polymerase
#P1061, 250U
Concentration: 5U/μl
Contents:
| Long Taq DNA Polymerase | 50 μl |
| 10× Long PCR Buffer Ⅰ (Mg2+ Plus) | 1.25 ml |
| 10× Long PCR Buffer Ⅱ (Mg2+ Plus) | 1.25 ml |
| PCR Enhancer | 500 μl |
| 6× Loading Buffer | 1 ml |
Store at -20°C
For research use only.
In total 5 vials.
Description
Long Taq DNA Polymerase, a combination of two thermostable DNA polymerases, Taq and Pfu, is a special formulation designed for amplifying long DNA fragments. This specially formulated Long Taq DNA Polymerase was shown to amplify long DNA templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying complex template, such as GC-rich template.
Long Taq DNA Polymerase is suitable as a replacement for ordinary Taq Polymerase in most applications. The elongation rate is 3kb/min. The products of Long Taq DNA Polymerase is a mix of 3´-dA overhangs and blunt-ends products, which can be used in TA clone.
Unit Definition
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.
Storage Buffer
20mM Tris-HCl (pH 8.0), 100mM KCl, 3mM MgCl2, 1mM DTT, 0.1% NP-40, 0.1% Tween20, 0.2mg/ml BSA, 50% (V/V) glycerol.
10× Long PCR Buffer Ⅰ with Mg2+
500mM Tris-HCl (pH 8.8), 160mM (NH4)2SO4, 25mM MgCl2, 1% Triton X-100.
10× Long PCR Buffer Ⅱ with Mg2+
200mM Tris-HCl (pH 8.8), 100mM KCl, 100mM (NH4)2SO4, 16mM MgSO4, 1% Triton X-100.
Note:
10× Long PCR Buffer Ⅰ is classical Long Taq DNA Polymerase buffer, and it’s good for long template DNA, especially above 10 kb.
10× Long PCR Buffer Ⅱ is a special buffer optimized by Dongsheng Biotech. It is for better fidelity but not good at long template DNA above 10 kb.
Users could choose suitable buffer for different template.
Features
• High fidelity: three times fidelity of Taq DNA Polymerase.
• Longer fragment: amplify long templates as long as 20 kb.
• Amplification of complex template (GC rich or repetitive
sequence).
• Generates 3'-dA and blunt-end PCR products.
Applications
• PCR amplification of complex template DNA
• PCR amplification of long DNA sequences
• DNA sequencing
• PCR for cloning
Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.
