Real Time SYBR Green Master Mix QPCR P2092 Colourless Appearance

Cat. No: P2091, 400 rxn/20μl reaction

P2092, 2000 rxn/20μl reaction

SYBR^® Green qPCR Mix

SYBR® Green qPCR Mix Real Time PCR Mix P2092

For research use only

Components

Storage

This reagent can be stored for 2 months at 4°C and protected from light. For longer storage, it should be kept at -20°C and protected from light.

Description

SYBR^® Green qPCR Mix is designed for high-performance, high-throughput real-time PCR. The kit contains Taq DNA Polymerase engineered through a process of molecular evolution. The result is a unique polymerase that specifically designed for qPCR using SYBR^® Green I chemistry dye.

2X SYBR^® Green qPCR Mix is a convenient premix of the components (except primers and DNA template) that necessary to perform real-time polymerase chain reaction (PCR) using SYBR^® Green I dye with enhanced sensitivity and specificity. The SYBR^® Green I dye binds to double-stranded DNA (dsDNA), thus providing a fluorescent signal that reflects the amount of dsDNA products generated during PCR.

Applications

• Gene expression analysis

• Low-copy gene detection

• Microarray validation

• Gene knockdown validation

Features

• Compatible with many Real-time systems which not require ROX reference dye

• Hot-start technology brings high specificity and reproducible amplification

Composition of the 2X SYBR Green qPCR Mix

100mM KCl , 5mM MgCl₂, 400μM dNTPs, 0.1U/μl Hotstart Taq DNA Polymerase, 1× SYBR^® Green I and other optimized buffer components.

Table of Instrument Guide

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.