High Specificity One Step RNA/DNA RT QPCR Master Mix With UDG V3

One-step Probe RT-qPCR Kit V3

High Specificity One-Step Probe RT-QPCR Kit V3 V5011 (200 Rxn 20μL/Rxn)

Cat. No.:V5011

Components

a. Contains dNTP/dUTP Mix, Mg²⁺.

b. Contains Reverse Transcriptase, RNase inhibitor, Heat-labile UDG and hot-start Taq DNA Polymerase.

Description

The One-step Probe RT-qPCR Kit V3 is designed for quantitative PCR detection using RNA as a template, such as viral RNA. Using gene-specific primers (GSP), reverse transcription and qPCR reactions are performed in a single tube, requiring no additional tube opening/pipetting, significantly increasing detection throughput and reducing the risk of contamination. This Kit introduced dUTP/UDG system. Heat-labile UDG can rapidly degrade the pollutants containing U at room temperature. When reverse transcription is performed at 55°C, heat-labile UDG will be inactivated rapidly without affecting the efficiency and sensitivity of RT-qPCR. Integrating the superior performance of Reverse Transcriptase and hot-start Taq DNA Polymerase, with the optimized buffer system, the detection sensitivity of One-step Probe RT-qPCR Kit can reach 0.1 pg total RNA or <10 copies of RNA template. The kit is available as a convenient Master Mix. 5 × One-step U⁺ Mix contains an optimized buffer system and dNTP/dUTP Mix, which is suitable for high-specificity, low template concentration and multiple detection systems with fluorescent labeled probes such as TaqMan^®. This product is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc.

Storage

This reagent should be kept at -20°C.

Applications

• Probe gene expression analysis

• Probe Low-copy gene detection

• Probe microarray validation

• Probe gene knockdown validation

Features

• One-step RT-qPCR

• This kit is suitable for fluorescence quantification by probe method

• This kit is compatible with many real-time systems

• Hot-start technology brings high specificity and reproducible amplification

• dUTP/UDG system, effectively prevent PCR product contamination

Notes

1. One-Step U⁺ Enzyme Mix V3 contains high concentration of glycerol. Before use, please centrifuge briefly, collect to the bottom of the reaction tube, and gently suck with a pipetting gun.

2. For preparation of reaction solution, please use RNase-free pipette tips, EP tubes, etc., to avoid contamination as far as possible.

GDSBio has a complete product line. With PCR technology as the core, focusing on molecular biology techniques such as ordinary PCR, fluorescence quantitative PCR, nucleic acid electrophoresis, nucleic acid purification, it has developed reagents for molecular biology research and diagnosis, and completed the layout of the whole industrial chain from molecular biology reagents, medical lab supplies to specialized services.