Active Member
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[China]
Address: Building D2, block A, Tri-medical innovation center, No.18, North Bayi Road, Yongning Town, Wenjiang District, Chengdu City, Sichuan Province, P.R.China
Contact name:inquiry
Foregene Co., Ltd. |
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Quickly purify and obtain high-quality genomic DNA from various soil sample microorganisms Soil DNA Isolation Kits
Description
This kit provides a quick and easy method for extracting genomic DNA from various sources of soil. There are a large number of inhibitors such as humic acid and metal ions in soil samples. Even if these substances are present in small amounts in the purified DNA, they will have an impact on downstream reactions, such as PCR and restriction enzyme digestion. Therefore, the key to purifying soil DNA is how to effectively remove inhibitors in the soil.
This kit uses DNA-Only Column that can specifically bind to DNA, a brand-new Foregene Protease and a unique buffer system, which can effectively remove various inhibitors from the soil without organic solvent extraction or ethanol and isopropanol precipitation. The extraction of DNA from soil samples can be completed in 90 minutes.
Kit conponents
| Soil DNA Isolation Kit | |||||
| Kit contents | DE-05511 | DE-05512 | DE-05513 | DE-05514 | DE-05515 |
| 5 T | 25 T | 50 T | 100T | 250T | |
| Buffer SG1 | 3.5ml | 18ml | 35ml | 70ml | 180ml |
| Buffer SG2 | 300μl | 1.5ml | 3ml | 6ml | 15ml |
| Buffer SG3 | 3.5ml | 18ml | 35ml | 70ml | 180ml |
| Buffer SG4 | 120μl | 600μl | 1.2ml | 2.4ml | 6ml |
| Buffer PW | 3ml | 15ml | 30ml | 60ml | 150ml |
| Buffer WB | 2.5ml | 12.5ml | 25ml | 50ml | 125ml |
| Buffer EB | 1.5ml | 6ml | 15ml | 60ml | 120ml |
| Buffer TE | 6ml | 30ml | 60ml | 120ml | 300ml |
| Foregene Protease | 280μl | 1.4ml | 1.4mlx2 | 5.6ml | 14ml |
| DNA-Only Column | 5 sets | 25 sets | 50 sets | 100 sets | 250 sets |
| IFU | 1 piece | 1 piece | 1piece | 1 piece | 1 piece |
Features&advantages
- No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove RNA from genomic DNA without adding RNase during the experiment, avoiding the laboratory from being contaminated by exogenous RNase.
- Fast speed: The operation is simple, and the soil genomic DNA extraction operation can be completed within 90 minutes.
- Convenient: The centrifugation is performed at room temperature, and there is no need for 4°C low-temperature centrifugation or ethanol precipitation of DNA.
- Safety: No organic reagent extraction is required.
- High quality: The extracted genomic DNA has large fragments, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.
Genomic DNA Application
The genomic DNA purified by Soil DNA Isolation Kit has high purity and can be used for routine molecular biology operations, such as: restriction enzyme digestion, PCR, Southern blotting, library construction and other experiments.
Quality Control
According to FOREGEN’s Total Quality Management System, each batch of Soil DNA Isolation kit is strictly tested multiple times to ensure the reliability and stability of the quality of each batch of kits.
DNA-Only Column Characters
| Maximum binding capacity | 80μg |
| Maximum loading volume | 800μl |
| Longset DNA fragment | 23kb |
| Minimum elution volume* | 100μl |
| Selection of samples | Newly taken soil samples |
| Maximum amount of starting material* | 5ml soil sample |
*: The minimum elution system of 100μl is a reasonable recommended volume considering the DNA recovery rate and concentration. If you want to increase the yield of DNA, you can increase the volume of the eluate; if you want to increase the concentration of the purified DNA, you can reduce the volume of the eluate appropriately, such as using a 50μl elution system, while sacrificing part of the DNA yield. In order to get a higher concentration of DNA.
Genomic DNA extraction yield and purity
The amount of soil genomic DNA extracted is related to factors such as the source, storage time, and moisture content of the soil sample. Process soil samples from various sources according to the Soil DNA Isolation Kit, and the amount and purity of genomic DNA obtained are shown in the following table:
| Sample source | Sample dosage(mg) | DNA yield(μg) | OD260/280 | OD260/230 |
| Flower bed soil | 500 | 4-5 | 1.7-1.9 | 1.7-1.9 |
| Grassland soil (stony) | 500 | 4-6 | 1.7-1.9 | 1.8-1.9 |
| Forest soil | 500 | 4-6 | 1.7-1.9 | 1.7-1.8 |
Lawn soil | 500 | 6-10 | 1.7-1.8 | 1.8-2.0 |
| Lakeside | 500 | 6-10 | ≈1.8 | 1.9-2.0 |
| Pine forest | 500 | 8-12 | ≈1.8 | 1.8-1.9 |
| Pond silt | 500 | 6-8 | 1.7-1.8 | 1.7-1.8 |
Note: The data in this table is for reference only. In actual operation, due to the storage conditions of the materials used, operating proficiency and other factors, the data obtained may differ slightly from the data in this table.
Storage and Shelf life
- This kit can be stored for 12 months under dry conditions at room temperature (15-25°C), if it needs to be stored for a longer period of time, it can be stored at 2-8°C.
Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to place the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.
- Foregene Protease solution has a unique formula, which is active when stored at room temperature for a long time (3 months); its activity and stability will be better when stored at 4°C, so it is recommended to store it at 4°C, remember not to keep it at -20°C.
- Dry Lysozyme powder is stored at -20°C; the prepared Lysozyme solution is divided into small portions and stored at -20°C.
