African Swine Fever Virus Antibodies Rapid Test Kit African Swine Fever Test Kit Animal Serum Diagnostic Rapid Tester

African Swine Fever Virus Antibody Rapid Test Kit

(Whole Blood / Serum / Plasma / Milk)

INTENDED USE

African Swine Fever Virus Antibody Rapid Test  (whole blood / serumr/ plasma/milk) is a solid phase immunochromatographic assay for the rapid,qualitative and dfferentia ldetection of antibody to animal whole blood serum, or plasma. This test provide only a preliminaryanalytical result. A secondary analytical method must be used to obtain a confirmed result. Gas chromatography and mass spectrometry (GC/MS) are the preferred confirmatory methods.

Naked eyes interpretation for Qualitative Results , Quantitative Analysis with machine.

PRINCIPLE

Quantitative or qualitative detection of the titer level of African porcine fever antibodies in samples using a double antigen sandwich method. During detection, the African swine fever antibodies in the sample binds to the colloidal gold-coated specific antigen fragment to form an antigen-antibody complex, which flows along the test paper to the other end of the NC membrane. When the complex flows to the T-zone on the membrane, the antigen immobilized on the membrane captures the complex and gradually agglutinates into a red visible T-line, while the unbound colloidal gold antibody flows through the T-zone and is captured by the secondary antibody in the C-zone and forms a visible red C-line.

KIT CONTENTS

STORAGE AND STABILITY

1. Store in a light-proof, dry environment (2-30℃).

2. DO NOT FREEZE or use beyond the expiration date.

3. The shelf life is 18 months.

QUALITATIVE ANALYSIS

1. Sample dilution to be tested: Pipette 35ml (or 1 drop) of serum sample, add to the sample dilution tube, gently invert up and down 10 times and mix thoroughly; The sample can be used for testing after dilution and can be stored for 1 day at room temperature.

2. Test card restored to room temperature: The test should be carried out at 20-30℃. If the test card is stored in a refrigerator (2-8℃), equilibrate it in a room temperature environment for 30 min before ripping the packaging inside the aluminum foil bag to avoid abnormal measurement results due to moisture absorption.

3. Test card number: After preparing the sample to be tested, remove the required test card and mark the sample number on the card.

4. Dropwise sample reaction for 20 min: Aspirate 70 ml of the serum sample to be tested, drop it into the sampling hole and leave it horizontally for 20 min.

READ RESULTS WITH NAKED EYES

NEGATIVE:

The Test Line (T) is the same as or darker than the Control Line(C). It is negative.

POSITIVE:

The Test Line (T) is lighter than the Control Line(C),or there is no Test Line. It is positive.

INVALID:Reference Line Fails to appear

Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for an invalid result.

Review the procedure and repeat the test with a new test kit. Stop using the test kit immediately if the problem

is not solved and contact your local distributor.