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REAGEN™ Aflatoxin M1 ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Aflatoxin M1 in cereals, meat/fish, feed, peanuts, pistachios cheese,milk powder and milk .
| Kit Contents | Amount | Storage |
| Aflatoxin M1 Antibody-Coated Plate | 1 x 96-well Plate(8 wells x 12 strips) | 2-8°C |
Aflatoxin M1 Standards: Negative control (white cap tube) 0.02ng/mL (yellow cap tube) 0.06ng/mL (orange cap tube) 0.18 ng/mL (pink cap tube) 0.54ng/mL (purple cap tube) 1.62ng/mL (blue cap tube) 10 ng/mL (spiking, red cap tube) |
0.5 mL 0.5 mL 0.5 mL 0.5 mL 0.5 mL 0.5 mL 0.5 mL |
2-8°C
2-8°C
|
| Aflatoxin M1 Antibody #1 | 6ml | 2-8°C
|
| HRP-Conjugated Antibody #2 | 11 mL | |
| 20X Wash Solution ** | 25 mL | |
| Stop Buffer ** | 11 mL | |
| TMB Substrate ** | 11 mL | |
| Milk Ext.Buffer I | 28 mLx3 | |
| 10x PBS | 10 mL | |
| Ext.Buffer A (optional) | 1.5 mL | |
| Ext.Buffer B (optional) | 1.5 mL | |
| Ext.Buffer C (optional) | 1.5 mL |
* If you are not planning to use the kit for over 3 months, store Aflatoxin M1 Antibody #1 and 1X HRP-Conjugated Antibody #2 at -20°C or in a freezer.
| Sample type | Detection Limit (ng/g or ppb) |
| Fresh milk | 0.03 |
| Milk powder | 0.1 |
| Yogurt | 0.05 |
| Whey powder | 0.1 |
| Condensed milk | 0.15 |
Analytes | Cross-Reactivity (%) |
Aflatoxin M1 | 100.0 |
Aflatoxin B1 | 36 |
Aflatoxin B2 | 25 |
Aflatoxin M2 | 19 |
Aflatoxin G1 | 17 |
Aflatoxin G2 | 3 |
1.High recovery (75-120%),rapid(10-15minutes), cost-effective extraction methods.
2.High sensitivity (0.02 ng/g or ppb) .
3.High reproducibility.
4.A quick ELISA assay (less than 1.5 hours regardless of number of samples).
5.Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The toxin of interest has been coated in plate wells. During the analysis, sample is added along with the primary antibody specific for target toxin. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the toxin attached to the well. The second antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the toxin coated on the wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
REAGEN™ Aflatoxin M1 ELISA Test Kit has the Aflatoxin M1acity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.
