Human Serum ELISA Assay 25- Hydroxyl Vitamin D Test Kit

25-Hydroxyl Vitamin D Test Kit

Product Description

REAGEN™ The 25-Hydroxyl Vitamin D Test Kit is suitable for the quantitative determination of 25-hydroxy vitamin D in human serum or plasma in vitro.

Procedure Overview

REAGEN™ The concentration of 25-hydroxy vitamin D in human serum was quantitatively determined by using fluorescence immunochromatography and competitive inhibition. The product detects the conjugate of 25-hydroxy vitamin D total antigen and bovine serum albumin,the quality control line is coated with goat anti-mouse IgG, and the fluorescent label tube contains anti-25-hydroxy vitamin D mouse monoclonal antibody-labeled fluorescent microspheres. ball. At the time of detection, if the sample contains 25-hydroxy vitamin D, an immune reaction with the antibody-labeled fluorescent microspheres will occur, thereby immunosuppressing the reaction between the labeled microspheres and the antigen on the detection line, and the fluorescence signal intensity of the detection line will be weakened accordingly. ; Whether or not the sample contains 25-hydroxy vitamin D, the microspheres will form a certain intensity fluorescent band in the quality control area.

The signal intensity of the band is negatively correlated with the concentration of 25-hydroxy vitamin D. The response signal of the quality control line and detection line of the 25-hydroxy vitamin D detection reagent is obtained by an immunofluorescence chromatography analyzer, and the measured signal can be generated through the measurement and analysis of the instrument. Quantitative test results of 25-hydroxy vitamin D in the sample.

Kit Contents, Storage and Shelf Life

Component

Quantity

10 servings / box

20 servings / box

Detection reagent

1 serving × 10

1 serving × 20

Fluorescent reaction solution

400μl / piece × 1 branch

800μl / piece × 1 branch

Precipitation release

400μl / piece × 1 branch

800μl / piece × 1 branch

Display board reaction solution

700μl / piece × 1 branch

1400μl / piece × 1 branch

Sample processing tube

10 pcs / bag × 1 bag

20 pcs / bag × 1 bag

ID card

1 sheet

1 sheet