Sirtuin 2 Elisa Test Kit , Mouse Ifn Gamma Elisa Kit Deacetylates
Lys-40 Alpha - Tubulin
Other names:SIR2L; SIR2-L; SIR2L2; Silent Information Regulator 2 Protein;
NAD-Dependent Deacetylase Sirtuin-2; Silent Mating Type Information
Regulation 2 Homolog
Function: NAD-dependent deacetylase, which deacetylates the ‘Lys-40′ of
alpha-tubulin. Involved in the control of mitotic exit in the cell
cycle, probably via its role in the regulation of cytoskeleton.
MAEPDPSDPL ETQAGKVQEA QDSDSDTEGG ATGGEAEMDF LRNLFTQTLG
LGSQKERLLD ELTLEGVTRY MQSERCRKVI CLVGAGISTS AGIPDFRSPS
TGLYANLEKY HLPYPEAIFE ISYFKKHPEP FFALAKELYP GQFKPTICHY
FIRLLKEKGL LLRCYTQNID TLERVAGLEP QDLVEAHGTF YTSHCVNTSC
RKEYTMGWMK EKIFSEATPR CEQCQSVVKP DIVFFGENLP SRFFSCMQSD
FSKVDLLIIM GTSLQVQPFA SLISKAPLAT PRLLINKEKT GQTDPFLGMM
MGLGGGMDFD SKKAYRDVAW LGDCDQGCLA LADLLGWKKE LEDLVRREHA
NIDAQSGSQA PNPSTTISPG KSPPPAKEAA RTKEKEEQQ
The kit is a sandwich enzyme immunoassay for the in vitro
quantitative measurement of SIRT2 in mouse serum, plasma, tissue
homogenates, cell lysates, cell culture supernates or other
1.56-100ng/mL. The standard curve concentrations used for the
ELISA’s were 100ng/mL, 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL,
The minimum detectable dose of SIRT2 is typically less than
The sensitivity of this assay, or Lower Limit of Detection (LLD)
was defined as the lowest protein concentration that could be
differentiated from zero. It was determined by adding two standard
deviations to the mean optical density value of twenty zero
standard replicates and calculating the corresponding
This assay has high sensitivity and excellent specificity for
detection of SIRT2.
No significant cross-reactivity or interference between SIRT2 and
analogues was observed.
Note: Limited by current skills and knowledge, it is impossible to
perform all possible cross-reactivity detection tests between SIRT2
and all analogues, therefore, cross reactivity may still exist.
Intra-assay Precision (Precision within an assay): 3 samples with
low, middle and high level SIRT2 were tested 20 times on one plate,
Inter-assay Precision (Precision between assays): 3 samples with
low, middle and high level SIRT2 were tested on 3 different plates,
8 replicates in each plate.
CV(%) = SD/meanX100
The stability of ELISA kit is determined by the loss rate of
activity. The loss rate of this kit is less than 5% within the
expiration date under appropriate storage conditions.
Note: To minimize unnecessary influences on the performance, operation
procedures and lab conditions, especially room temperature, air
humidity, and incubator temperatures should be strictly regulated.
It is also strongly suggested that the whole assay is performed by
the same experimenter from the beginning to the end.
ASSAY PROCEDURE SUMMARY
1. Prepare all reagents, samples and standards;
2. Add 100L standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100L prepared Detection Reagent A. Incubate 1
hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100L prepared Detection Reagent B. Incubate 1 hour at 37oC;
6. Aspirate and wash 5 times;
7. Add 90L Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50L