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Sirtuin 2 Elisa Test Kit , Mouse Ifn Gamma Elisa Kit Deacetylates Lys-40 Alpha - Tubulin

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Sirtuin 2 Elisa Test Kit , Mouse Ifn Gamma Elisa Kit Deacetylates Lys-40 Alpha - Tubulin

Country/Region china
City & Province wuxi jiangsu

Product Details

Sirtuin 2 Elisa Test Kit , Mouse Ifn Gamma Elisa Kit Deacetylates Lys-40 Alpha - Tubulin

Product Detail

Product Tags

1. Overview

Other names:SIR2L; SIR2-L; SIR2L2; Silent Information Regulator 2 Protein; NAD-Dependent Deacetylase Sirtuin-2; Silent Mating Type Information Regulation 2 Homolog

Function: NAD-dependent deacetylase, which deacetylates the ‘Lys-40′ of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton.

Sequence:

MAEPDPSDPL ETQAGKVQEA QDSDSDTEGG ATGGEAEMDF LRNLFTQTLG

LGSQKERLLD ELTLEGVTRY MQSERCRKVI CLVGAGISTS AGIPDFRSPS

TGLYANLEKY HLPYPEAIFE ISYFKKHPEP FFALAKELYP GQFKPTICHY

FIRLLKEKGL LLRCYTQNID TLERVAGLEP QDLVEAHGTF YTSHCVNTSC

RKEYTMGWMK EKIFSEATPR CEQCQSVVKP DIVFFGENLP SRFFSCMQSD

FSKVDLLIIM GTSLQVQPFA SLISKAPLAT PRLLINKEKT GQTDPFLGMM

MGLGGGMDFD SKKAYRDVAW LGDCDQGCLA LADLLGWKKE LEDLVRREHA

NIDAQSGSQA PNPSTTISPG KSPPPAKEAA RTKEKEEQQ

2. Features

INTENDED USE

The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of SIRT2 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

DETECTION RANGE

1.56-100ng/mL. The standard curve concentrations used for the ELISA’s were 100ng/mL, 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.12ng/mL, 1.56ng/mL.

SENSITIVITY

The minimum detectable dose of SIRT2 is typically less than 0.61ng/mL.

The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

SPECIFICITY

This assay has high sensitivity and excellent specificity for detection of SIRT2.

No significant cross-reactivity or interference between SIRT2 and analogues was observed.

Note: Limited by current skills and knowledge, it is impossible to perform all possible cross-reactivity detection tests between SIRT2 and all analogues, therefore, cross reactivity may still exist.

PRECISION

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SIRT2 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SIRT2 were tested on 3 different plates, 8 replicates in each plate.

CV(%) = SD/meanX100

Intra-Assay: CV<10%

Inter-Assay: CV<12%

STABILITY

The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.

Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

ASSAY PROCEDURE SUMMARY

1. Prepare all reagents, samples and standards;

2. Add 100L standard or sample to each well. Incubate 2 hours at 37oC;

3. Aspirate and add 100L prepared Detection Reagent A. Incubate 1 hour at 37oC;

4. Aspirate and wash 3 times;

5. Add 100L prepared Detection Reagent B. Incubate 1 hour at 37oC;

6. Aspirate and wash 5 times;

7. Add 90L Substrate Solution. Incubate 15-25 minutes at 37oC;

8. Add 50L

 

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