Triiodothyronine(T3) ELISA Test Kit
INTENDED USE
Detection Kit for 3,5,3'-Triiodothyronine(T3)(Enzyme-Linked ImmunoSorbent Assay , ELISA)It is used in
quantitative tests for 3,5,3’-triiodothyronine (T3) in human
serum.) in human serum.
SUMMARY AND CLINICAL SIGNIFICANCE
The thyroid gland is essential for the development of many
tissues and for the adjustement of their metabolism. These
processes are under the control of thyroid hormones, T4 and T3.
Under stimulation by pituitary thyrotropin(TSH). the thyroid gland
essentially secretes T4 with minute amounts of T3.
Tissue recptors to thyroid hormones are T3 receptors, T3 represents thus the acive entity among thyroid secretions.
The bulk of circulating T3 is produced by peripheral deiodination of T4 alternatively, T4 yields reverse T3(or RT3), a poorly active thyronine.
In the blood,T3 circulates free and bound to the same carrier protein transporting
T4, thyroxine binding globulin or TBG. Nevertheless, T3 binding affinity to TBG is significantly less than that of T4; The free T3 serum concentration represents about 0.3% of the total circulating
T3 concentration.
Under normal conditions, total T3 level is negatively. Correlated to serum TSH level due to the
negative feed -back mechanism regulating thyroid funcion.
Product list
Product details | Description |
Delivery | Within 48 hours |
Packaging Specifications | 8 x 12 strips, 96 wells |
Country Of Origin | China |
Manufacturer | 18 months |
Preservation method | 2℃-8℃ |
Specimen | Whole blood |
Assification | class1 |
Type | Elisa Test Kit |
PRINCIPLE
T3 kit is based on competitive method. Coat micro well plates with
anti-T3 to form solid-phase antibody. The T3 of sample and
biotin-T3 compet with anti-T3 on solid-phase. Vidin-HRP added,
solid phase antibody-biotinylated T3-avidin-HRP complex was formed.
Add substrate and detect absorbent value. Calculate T3 content of
patient serum through computer or plotting fitting
concentration-absorbent value curve.
MAIN COMPONENTS
- Coated Microplate: 1 plate (8×12 wells), Ready to use. Coated with
anti-T3 antibody and sealed in an aluminum bag. Remove the strips
in the resealable bag with a desiccant to protect from moisture
after opened. Store at 2~8℃ until expiration date.
- Biotin-T3: 1 vial of 6ml, Ready to use. Store at 2~8℃ until
expiration date.
- Avidin-HRP Conjugate: 1 vial of 6ml, Ready to use. Store at 2~8℃
until expiration date.
- Calibrator: 6 vials of 1ml, Ready to use. Labeled with S0 to S5 and the concentration of T3 is 0,0.5,1.0,2.0,4.0,8.0 ng/ml. Store
at 2~8℃ until expiration date.
- Control: 2 vials of 1ml, Ready to use. Store at 2~8℃ until
expiration date. The concentration of low value is 0.63~1.17ng/ml.
The concentration of high value is 2.8~5.2 ng/ml.
- Chromogen A: 1 vial of 7ml, Ready to use. Store at 2~8℃ until
expiration date.
- Chromogen B: 1 vial of 7ml, Ready to use. Store at 2~8℃ until
expiration date.
- Stop Solution: 1 vial of 7ml, Ready to use. Store at 2~8℃ until
expiration date.
- Wash buffer: 1 vial of 15ml, Concentrate 20-fold, diluting with
deionized water before the assay. Store at 2~8℃ until expiration
date.
- Plate sealer: 2 pieces.
- Plastic resealable bag: 1 set.
- Instruction manual: 1 copy.
RELATED TIPS
- This kit is designated for In-Vitro Diagnostic Use Only.
- Wash procedure. Incomplete washing will adversely affect the test
results. Wash each well 3 times with about 0.3ml wash buffer. If no
automatic washer is available, washing can be performed manually as
follows: Invert the plate vigorously to get all wash buffer out and
block the rim of well on absorbent paper for a few seconds. Filling
each well with water and remain 10 seconds. Repeat these steps 3
times. Blot dry the plate by inverting the plate onto absorbent
tissue, and striking a hard surface several times.
- Drip procedure. Mix the bottle gently before use. Violent surge may
cause too much foam. Invert the bottle and squeeze one or two drop
on absorbent tissue to make sure there is no foam. Take the bottle
upright the well and make sure the drop does not touch the rim of
wells.
- Read procedure. Using the blank well to correct the zero point of
reader if single wavelength reader is used. If double wavelength
readers with 450nm and 630nm are used, there is no need to correct
the zero point.
Storage. The whole kit should be stored at 2~8℃ for one year.
Microplate should be
PREPARATIONS
1. Allow all specimens and reagents to reach room temperature
(~25°C) and mix thoroughly by gentle inversion before use.
2.Prepare Wash Buffer by diluting Wash Concentrate 20-fold with
deionized water. The diluted wash solution is stable in room
temperature for at least one week.
ATTENTIONS
- This kit is designated for In-Vitro Diagnostic Use Only.
- Wash procedure. Incomplete washing will adversely affect the test
results. Wash each well 3 times with about 0.3ml wash buffer. If no
automatic washer is available, washing can be performed manually as
follows: Invert the plate vigorously to get all wash buffer out and
block the rim of well on absorbent paper for a few seconds. Filling
each well with water and remain 10 seconds. Repeat these steps 3
times. Blot dry the plate by inverting the plate onto absorbent
tissue, and striking a hard surface several times.
- Drip procedure. Mix the bottle gently before use. Violent surge may
cause too much foam. Invert the bottle and squeeze one or two drop
on absorbent tissue to make sure there is no foam. Take the bottle
upright the well and make sure the drop does not touch the rim of
wells.
- Read procedure. Using the blank well to correct the zero point of
reader if single wavelength reader is used. If double wavelength
readers with 450nm and 630nm are used, there is no need to correct
the zero point.
- Storage. The whole kit should be stored at 2~8℃ for one year.
Microplate should be taken to room temperature before opened. This
is very important because absorbed atmospheric moisture by cold
plates significantly reduces their shelf life. After removing the
required number of strips, the plate should be put in the plastic
resealable bag with
desiccants to minimize exposure to damp air.
- Control serum is prepared with human serum, which is tested
negative of HBV, HCV and HIV. But it should still be considered as
capable of transmitting viral diseases.
PRECAUTION FOR USERS
1. Handling should preclude any pipetting by mouth. Use only
pipettes with disposable tips for each specimen.
2.Do not mix materials from different master lots. Do not use kit
components beyond the expiration date.
3.All materials should be brought to room temperature before use.