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Agarose NHS Magbeads Preactivated N - Hydroxy Succinimide
Nanoparticle 10 - 30 μm, 20% (v/v)
1, Description:
BeaverBeads ™ Mag NHS and BeaverBeads ™ Magrose NHS are spherical
beads modified with NHS groups capable of forming stable peptide
bonds with proteins and other molecules with primary amine groups
for affinity purification or assay detection of antibodies,
antigens and other biomolecules. Compared with the traditional
beads with carboxyl or amino groups, the beads with surface
containing NHS group do not need to be activated by EDC / NHS or
glutaraldehyde in advance. Simply by dissolving the ligand
containing the primary amino acid in the coupling buffer (mix the
protein solution with Mag NHS beads at room temperature for 1 ~
2h), the ligands will be attached on the beads.
2, Introduction:
| Product Name | Agarose NHS Magbeads Preactivated |
| Mean particle size | 10-30 μm |
| Magnetic core | Fe3O4 |
| Shell | Agarose |
| Magnetic | superparamagnetism |
| Ligand content | ~50 μM/mL gel |
| Binding ability | 20~30 mg rabbit IgG/mL beads |
| Preservation fluid | 20% (v/v) ethanol solution |
| Operation | Automate/manual |
| * water average particle size, Malvern Nano determination | |
3, FEATURES:
1. Simple, without activation, can be directly covalently coupled with the biological ligand;
2. Efficient, bio-ligand coupling efficiency of up to 90% or more, much higher than the carboxyl beads, while the ligand with a higher binding capacity;
3. Fast, 1 ~ 2h to complete the bio-ligand coupling;
4. Mild, room temperature or 4 ℃ coupling, coupling system pH 5 ~ 9;
5. Stable, the formation of a stable amide bond, to prevent the ligand off.
6. Good biocompatibility, reduced nonspecific adsorption.
